A marker-free automatic alignment method based on scale-invariant features
A marker-free automatic alignment method based on scale-invariant features
复制标题
一种基于尺度不变特征的无标记自动对齐方法
DOI:
10.1016/j.jsb.2014.02.011
复制
发表时间:
2014-04-01
影响因子:
3
通讯作者:
Liu, Zhiyong
中科院分区:
文献类型:
--
作者:
Han, Renmin;Zhang, Fa;Liu, Zhiyong
In electron tomography, alignment accuracy is critical for high-resolution reconstruction. However, the automatic alignment of a tilt series without fiducial markers remains a challenge. Here, we propose a new alignment method based on Scale-Invariant Feature Transform (SIFT) for marker-free alignment. The method covers the detection and localization of interest points (features), feature matching, feature tracking and optimization of projection parameters. The proposed method implements a highly reliable matching strategy and tracking model to detect a huge number of feature tracks. Furthermore, an incremental bundle adjustment method is devised to tolerate noise data and ensure the accurate estimation of projection parameters. Our method was evaluated with a number of experimental data, and the results exhibit an improved alignment accuracy comparable with current fiducial marker alignment and subsequent higher resolution of tomography. (C) 2014 Published by Elsevier Inc.