Chryseobacterium shandongense sp nov., isolated from soil
Chryseobacterium shandongense sp nov., isolated from soil
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山东金黄杆菌
DOI：
10.1099/ijs.0.000186
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发表时间：
2015-06-01
影响因子：
2.8
通讯作者：
Hong, Qing
中科院分区：
文献类型：
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作者：
Yang, Fan;Liu, Hong-ming;Hong, Qing
YF-3(T)是一株革兰氏阴性杆菌，不能移动，不形成芽胞，黄色橙色，杆状。最适生长条件为30℃、pH 7.0、10/0(w/v)的氯化钠。根据16S rRNA基因序列进行的系统发育分析表明，YF-3(T)与金杆菌AG13(T)和台湾金杆菌Soil-3-27(T)的同源性分别为98.71%和96.93%。菌株YF-3(T)以MK-6为主要成分，具有以均三胺为主的多胺构型。其主要极性脂为磷脂酰乙醇胺。菌株YF-3(T)的主要脂肪酸是iso-C-15：0、iso-C-17：03-OH、总和特征9(包括iso-C-17：1 omega 9C和/或C-16：0 10-甲基)和总和特征3(包括C-16：1 omega 7C和/或C-16：1 omega 6C)。菌株YF-3(T)的DNA G+C含量为37mol%。YF-3(T)菌株与最近亲缘关系较近的三个菌株间的DNA-DNA关联度分别为31.7+/-2.1%和28.4+/-5.4%。在此基础上，发现了一个新种，命名为山东金杆菌。11月是提议的。模式菌株为YF-3(T)(=CCTCC AB 2014060(T)=JCM 30154(T))。
YF-3(T) is a Gram-stain-negative, non-motile, non-spore-forming, yellow orange, rod-shaped bacterium. Optimal growth conditions were at 30 degrees C, pH 7.0 and 10/0 (w/v) NaCl. Phylogenetic analysis, on the basis of the 16S rRNA gene sequence, showed that strain YF-3(T) was closely related to the strains Chryseobacterium hispalense AG13(T) and Chryseobacterium taiwanense Soil-3-27(T) with 98.71 % and 96.93 % sequence similarity, respectively. Strain YF-3(T) contained MK-6 as the main menaquinone and had a polyamine pattern with sym-homospermidine as the major component. Its major polar lipid was phosphatidylethanolamine. The dominant fatty acids of strain YF-3(T) were iso-C-15:0, iso-C-17:0 3-OH, summed feature 9 (comprising iso-C-17:1 omega 9c and/or C-16:0 10-methyl) and summed feature 3 (comprising C-16 :1 omega 7C and/or C-16:1 omega 6C). The DNA G + C content of strain YF-3(T) was 37 mol%. The DNA-DNA relatedness levels between strain YF-3(T) and the most closely related strains, C. hispalense AG13(T) and C. taiwanense Soil-3-27(T), were 31.7 +/- 2.1 % and 28.4 +/- 5.4 %, respectively. Based on these results, a novel species named Chryseobacterium shandongense sp. nov. is proposed. The type strain is YF-3(T) (= CCTCC AB 2014060(T) = JCM 30154(T)).