Flux of fatty acids during epidermal differentiation.
Flux of fatty acids during epidermal differentiation.
复制标题
表皮分化过程中脂肪酸的流动。
DOI：
10.1111/1523-1747.ep12510328
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发表时间：
1977
期刊：
影响因子：
--
通讯作者：
T. Traczyk
中科院分区：
文献类型：
--
作者：
R. Freinkel;T. Traczyk
在体外用[14C]亚油酸预标记角化前（妊娠18天）的胎鼠皮肤，随后在器官培养中进行分化和角化。在48小时的培养总表皮14C脂肪酸和14C二烯减少磷脂和增加甘油三酯和甾醇酯的颗粒层和角质层形成。在第二个24小时期间，没有从表皮中的14 C脂肪酸的净损失，这表明磷脂和中性酯的变化代表角质化细胞内的脂肪酸易位。研究结果是一致的假设，即通过磷脂水解释放的脂肪酸被打捞的中性脂质酯在表皮角质化的再酯化。
Fetal rat skin prior to cornification (18 days of gestation) was prelabeled with [14C] linoleic acid in vitro and subsequently allowed to differentiate and cornify in organ culture. During 48 hr in culture total epidermal 14C fatty acids and 14C dienes decreased in phospholipids and increased in triglycerides and sterol esters as granular layers and stratum corneum formed. During the second 24-hr period, there was no net loss in 14C fatty acids from the epidermis, suggesting that the changes in phospholipid and neutral esters represented translocations of fatty acids within the cornifying cells. The findings are consistent with the hypothesis that fatty acids liberated by hydrolysis of phospholipids are salvaged by reesterfication as neutral lipid esters during epidermal cornification.