CENTRIPETAL TRANSPORT OF CYTOPLASM, ACTIN, AND THE CELL-SURFACE IN LAMELLIPODIA OF FIBROBLASTS
CENTRIPETAL TRANSPORT OF CYTOPLASM, ACTIN, AND THE CELL-SURFACE IN LAMELLIPODIA OF FIBROBLASTS
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DOI：
10.1002/cm.970110403
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发表时间：
1988-01-01
影响因子：
--
通讯作者：
TAYLOR, DL
中科院分区：
文献类型：
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作者：
FISHER, GW;CONRAD, PA;TAYLOR, DL
用视频增强对比（VEC）显微镜研究了瑞士3 T3培养物中的伤口愈合。在伸展阶段，通常持续1至4小时伤后的细胞沿着伤口的前缘突起的形成进行了详细的研究。板状伪足表现出连续向后或向心运输的各种细胞成分的速率约。0.26从前缘开始以μ m/sec的速度移动。片状伪足也是肌动蛋白微刺侧向迁移和伸缩的部位。还观察到横向于运动方向的肌动蛋白纤维以类似的速率向心运输。这些纤维可能部分上升为大肌动蛋白纤维，形成在板状伪足基部和板状伪足之间的界面处。附着于板状伪足背表面的直径为0.5 μ m的珠粒也以约0.5 μ m的速率向心运输。0.21μ m/sec。因此，有一个明显的相关性运输的各种结构内板状伪足和表面运动的板状伪足。
Wound healing in Swiss 3T3 cultures was investigated with video-enhanced contrast (VEC) microscopy. The formation of protrusions at the leading edge of cells along wounds was investigated in detail during the spreading stage, which usually lasted from 1 to 4 hr postwounding. Lamellipodia exhibited a continuous rearward, or centripetal, transport of a variety of cellular constituents at rates of .apprx. 0.26 .mu.m/sec from the leading edge. The lamellipodia were also the sites of lateral migration as well as extension and retraction of actin microspikes. Actin fibers oriented transversely to the direction of movement were also observed to transport centripetally at similar rates. These fibers may in part rise to large actin fibers forming at the interface between the base of the lamellipodia and the lamellae. Beads 0.5 .mu.m in diameter attached to the dorsal surfaces of lamellipodia also transported centripetally at rates of .apprx. 0.21 .mu.m/sec. Thus there is an apparent correlation between transport of a variety of structures within lamellipodia and with surface movements of lamellipodia.