Wnt11 facilitates embryonic stem cell differentiation to Nkx2.5-positive cardiomyocytes
Wnt11 facilitates embryonic stem cell differentiation to Nkx2.5-positive cardiomyocytes
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DOI：
10.1016/j.bbrc.2004.10.103
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发表时间：
2004-12-17
影响因子：
3.1
通讯作者：
Morisaki, T
中科院分区：
文献类型：
--
作者：
Terami, H;Hidaka, K;Morisaki, T
Wnt信号在多种组织的形态发生调控中起重要作用。在此，我们描述了Wnt11在胚胎干细胞心脏分化过程中的作用。首先，我们检测了Wnt11在胚状体分化过程中的表达谱，然后比较了其在维甲酸处理和未处理的胚状体中的表达。在胚状体分化过程中，Wnt11的表达随Nkx2.5的表达而升高，并持续升高。当视黄酸处理胚胎样体时，Wnt11的表达与心脏基因的表达同时下降。此外，用含有Wnt11的培养基处理胚状体增加了心脏标记基因的表达。基于这些结果，我们提出Wnt11在胚胎样体心脏发育中起重要作用，可能是心脏发育过程中心肌细胞增殖和分化的关键调节因子。(C) 2004爱思唯尔公司版权所有。
Wnt signaling plays a crucial role in the control of morphogenesis in several tissues. Herein, we describe the role of Wnt11 during cardiac differentiation of embryonic stem cells. First, we examined the expression profile of Wnt11 during the course of differentiation in embryoid bodies, and then compared its expression in retinoic acid-treated embryoid bodies with that in untreated. In differentiating embryoid bodies, Wnt11 expression rose along with that of Nkx2.5 expression and continued to increase. When the embryoid bodies were treated with retinoic acid, Wnt11 expression decreased in parallel with the decreased expression of cardiac genes. Further, treatment of embryoid bodies with medium containing Wnt11 increased the expression of cardiac marker genes. Based on these-results, we propose that Wnt11 plays an important role for cardiac development by embryoid bodies, and may be a key regulator of cardiac muscle cell proliferation and differentiation during heart development. (C) 2004 Elsevier Inc. All rights reserved.