Molecular cloning and expression of a human B-cell growth factor gene in Escherichia coli.
Molecular cloning and expression of a human B-cell growth factor gene in Escherichia coli.
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人 B 细胞生长因子基因在大肠杆菌中的分子克隆和表达。
DOI：
10.1126/science.3547651
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Maizel,A
中科院分区：
文献类型：
--
作者：
Sharma,S;Mehta,S;Morgan,J;Maizel,A
人B细胞生长因子（BCGF）（12千道尔顿）支持B淋巴细胞的克隆增殖。分离出一个克隆，它含有在大肠杆菌中编码具有生物活性的12千道尔顿BCGF的适当结构序列，并与通过在非洲爪蟾卵母细胞中体外翻译鉴定的特异性信使RNA杂交。在124个氨基酸长的多肽的氨基末端发现了18个氨基酸的相对疏水区域。羧基末端由至少32个氨基酸组成，所述氨基酸衍生自与Alu重复家族具有显著同源性的核苷酸序列。
A human B-cell growth factor (BCGF) (12 kilodaltons) supports the clonal proliferation of B lymphocytes. A clone was isolated that contained the proper structural sequence to encode biologically active, 12-kilodalton BCGF inEscherichia coliand to hybridize to a specific messenger RNA, identified by in vitro translation inXenopus laevisoocytes. A relatively hydrophobic region of 18 amino acids was found at the amino terminal of the 124-amino acid-long polypeptide. The carboxyl terminal is composed of at least 32 amino acids that are derived from nucleotide sequences bearing significant homology to the Alu repeat family.