Isolation and characterization of isopiperitenol dehydrogenase from piperitenone-type Perilla.
Isolation and characterization of isopiperitenol dehydrogenase from piperitenone-type Perilla.
复制标题
胡椒烯酮型紫苏中异胡椒烯醇脱氢酶的分离和表征。
DOI：
10.1248/bpb.b14-00090
复制
发表时间：
2014
影响因子：
2
通讯作者：
Michiho Ito
中科院分区：
文献类型：
--
作者：
Naoko Sato;Michiho Ito
研究紫苏油类化合物的生物合成将有助于阐明次生代谢物的调控系统和天然产物合成的反应机理。在本研究中，从紫苏的三个纯系中分离到两种类型的乙醇脱氢酶，即异戊二烯醇脱氢酶1和2(ISPD1和ISPD2)，它们被认为参与了紫苏生物合成中异戊二烯醇氧化的过程。ISPD1和ISPD2都与氧化形式的烟酰胺腺嘌呤二核苷酸(NAD(+))辅因子一起将异戊二烯醇氧化成异匹马烯酮。ISPD1使用两种异戊二烯醇非对映异构体，而ISPD2使用顺式异构体作为底物。然而，从胡椒烯酮型紫苏中只分离到ISPD2。这些结果表明，在紫苏中，ISPD2与胡椒烯酮的生物合成有关，胡椒酮是由(-)-顺-异戊二烯醇形成的。
Studying the biosynthesis of oil compounds in Perilla will help to elucidate regulatory systems for secondary metabolites and reaction mechanisms for natural product synthesis. In this study, two types of alcohol dehydrogenases, isopiperitenol dehydrogenases 1 and 2 (ISPD1 and ISPD2), which are thought to participate the oxidation of isopiperitenol in the biosynthesis of perilla, were isolated from three pure lines of perilla. Both ISPD1 and ISPD2 oxidized isopiperitenol into isopiperitenone with an oxidized form of nicotinamide adenine dinucleotide (NAD(+)) cofactor. ISPD1 used both isopiperitenol diastereomers, whereas ISPD2 used cis-isomer as a substrate. However, only ISPD2 was isolated from piperitenone-type perilla. These results suggests that in perilla, ISPD2 is related to the biosynthesis of piperitenone, which was formed via (-)-cis-isopiperitenol.
紫苏 4S-(-)-柠檬烯合酶的 cDNA 克隆、表征和功能表达。
DOI：
10.1006/abbi.1996.0343
发表时间：
1996
期刊：
Archives of biochemistry and biophysics.
影响因子：
--
作者：
Yuba,A;Yazaki,K;Tabata,M;Honda,G;Croteau,R
通讯作者：
Croteau,R