Human seroreactivity to gut microbiota antigens
Human seroreactivity to gut microbiota antigens
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DOI：
10.1016/j.jaci.2015.03.036
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发表时间：
2015-11-01
影响因子：
14.2
通讯作者：
Elson, Charles O.
中科院分区：
文献类型：
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作者：
Christmann, Benjamin S.;Abrahamsson, Thomas R.;Elson, Charles O.
背景：虽然在某些疾病中可以观察到针对肠道微生物区系抗原的免疫反应，但人类对肠道微生物区系的正常适应性免疫反应并不明确。目的：评估143名健康成年人对肠道微生物区系的获得性免疫反应，并将这种反应与52名患有变态反应性疾病的儿童及其母亲的观察到的反应进行比较。方法：采集成人和儿童从出生到7岁的血清，使用一种新的蛋白质微阵列来评估血清对一组肠道微生物区系抗原的免疫应答。结果：几乎每个受试者，无论健康状况如何，都可以观察到对一组肠道微生物区系抗原的血清免疫应答。结果：几乎每个受试者，无论健康状况如何，有能识别一组常见抗原的血清免疫球蛋白。在特应性成人中，对抗原面板的血清反应性显著降低。健康婴儿对肠道微生物区系抗原的免疫球蛋白血清反应性最高。这种适应性反应在6到12个月龄之间发展，并在2岁左右达到顶峰。婴儿时期对某些微生物群抗原的低免疫球蛋白反应与儿童时期过敏的发生有关。结论：在过敏性受试者中观察到对微生物群抗原的适应性反应的扰动。这些扰动甚至在儿童时期就可以观察到，这表明微生物群对适应性免疫系统的最佳刺激可能是预防某些免疫介导性疾病所必需的。
Background: Although immune responses directed against antigens from the intestinal microbiota are observed in certain diseases, the normal human adaptive immune response to intestinal microbiota is poorly defined.Objective: Our goal was to assess the adaptive immune response to the intestinal microbiota present in 143 healthy adults and compare this response with the response observed in 52 children and their mothers at risk of having allergic disease.Methods: Human serum was collected from adults and children followed from birth to 7 years of age, and the serum IgG response to a panel of intestinal microbiota antigens was assessed by using a novel protein microarray.Results: Nearly every subject tested, regardless of health status, had serum IgG that recognized a common set of antigens. Seroreactivity to the panel of antigens was significantly lower in atopic adults. Healthy infants expressed the highest level of IgG seroreactivity to intestinal microbiota antigens. This adaptive response developed between 6 and 12 months of age and peaked around 2 years of age. Low IgG responses to certain clusters of microbiota antigens during infancy were associated with allergy development during childhood.Conclusions: There is an observed perturbation of the adaptive response to antigens from the microbiota in allergic subjects. These perturbations are observable even in childhood, suggesting that optimal stimulation of the adaptive immune system by the microbiota might be needed to prevent certain immune-mediated diseases.