Rapid decline in sputum IL-10 concentration following occupational smoke exposure
Rapid decline in sputum IL-10 concentration following occupational smoke exposure
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DOI：
10.1080/089583701753403953
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发表时间：
2002-02-01
影响因子：
2.1
通讯作者：
Lantz, RC
中科院分区：
文献类型：
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作者：
Burgess, JL;Nanson, CJ;Lantz, RC
烟雾暴露对炎症介质如白细胞介素-10（IL-10）、白细胞介素-8（IL-8）和肿瘤坏死因子-α（TNF-α）的急性影响尚未完全了解。我们的研究旨在测量低水平烟雾暴露后消防员痰液中这些细胞因子的浓度。在基线时，参与的消防员进行了血液收集，肺功能测试，并通过吸入高渗盐水诱导痰。研究参与者后来对结构火灾进行了大修，在此期间，他们戴着弹药筒灭火器，并监测烟雾暴露情况。检修包括寻找和消除隐藏的燃烧源。大修后1小时，再次采集血液、肺功能数据和诱导痰。通过酶联免疫吸附试验（ELISA）测定痰液上清液中的IL-10、IL-8和TNF-α浓度。在17名消防员中，基线痰IL-10浓度为57.0+/-56.8 pg/L，下降至16.9+/-27。大修后2 pg/L（p=.02）。未观察到痰液IL-8和TNF-α浓度的显著变化。大修后，研究参与者的用力肺活量（FVC）显著下降。血清中的克拉拉细胞蛋白和表面活性剂相关蛋白A的浓度显着增加大修后，表明肺通透性增加。IL-10浓度似乎对烟雾非常敏感，痰中IL-10的研究应控制近期暴露。IL-10对炎症的抑制作用降低可能是低水平烟雾暴露导致肺损伤的机制。
The acute effects of smoke exposure on inflammatory mediators such as interleukin-10 (IL-10), interleukin-8 (IL-8), and tumor necrosis factor-alpha(TNF-alpha) are not well understood. Our study was designed to measure sputum concentrations of these cytokines in firefighters following low-level smoke exposure. At baseline, participating firefighters underwent blood collection, pulmonary function testing, and sputum induction through inhalation of nebulized hypertonic saline. Study participants later performed overhaul of a structural fire, during which time they wore cartridge respirators and were monitored for smoke exposure. Overhaul involves searching for and extinguishing hidden sources of combustion. One hour following overhaul, blood, pulmonary function data, and induced sputum were again collected. IL-10, IL-8, and TNF-alpha concentrations were measured by enzyme-linked immunosorbent assay (ELISA) in sputum supernatant. In 17 firefighters, baseline sputum IL-10 concentrations were 57.0+/-56.8 pg/L, and declined to 16.9+/-27. 2 pg/L following overhaul (p=.02). No significant changes were observed in sputum IL-8 and TNF-alpha concentrations. Forced vital capacity (FVC) declined significantly in study participants following overhaul. Serum concentrations of Clara-cell protein and surfactant-associated protein A increased significantly following overhaul, indicating increased lung permeability. IL-10 concentrations appear to be exquisitely sensitive to smoke, and studies of IL-10 in sputum should control for recent exposure. Reduced suppression of inflammation by IL-10 may be a mechanism by which low-level smoke exposure causes lung injury.