Analysis of flagellar size control using a mutant of Chlamydomonas reinhardtii with a variable number of flagella.
Analysis of flagellar size control using a mutant of Chlamydomonas reinhardtii with a variable number of flagella.
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DOI：
10.1083/jcb.92.1.170
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发表时间：
1982-01
期刊：
影响因子：
--
通讯作者：
Jarvik JW
中科院分区：
文献类型：
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作者：
Kuchka MR;Jarvik JW
每个细胞鞭毛数量可变的莱茵衣藻突变体已被用于研究鞭毛大小控制。突变型和野生型在细胞大小和鞭毛长度上没有差异，但单个突变细胞的细胞内鞭毛前体蛋白库的大小可能显着不同，例如，三鞭毛细胞的库大小是单鞭毛细胞库的三倍。由于大小相同但池大小差异很大的细胞具有相同长度的鞭毛，因此未组装的鞭毛前体蛋白池的浓度似乎并不调节鞭毛长度。对于不同大小和倍性的野生型细胞，还研究了细胞大小、库大小和鞭毛长度之间的关系。同样，鞭毛长度似乎与池大小或浓度无关。
A mutant of Chlamydomonas reinhardtii with a variable number of flagella per cell has been used to investigate flagellar size control. The mutant and wild-type do not differ in cell size nor in flagellar length, yet the size of the intracellular pool of flagellar precursor protein can differ dramatically among individual mutant cells, with, for example, triflagellate cells having three times the pool of monoflagellate cells. Because cells of the same size, but with very different pool sizes, have flagella of identical length, it appears that the concentration of the unassembled flagellar precursor protein pool does not regulate flagellar length. The relation between cell size, pool size, and flagellar length has also been investigated for wild-type cells of different sizes and ploidies. Again, flagellar length appears to be maintained independent of pool size or concentration.