Mechanisms of Molecular Mimicry of Plant CLE Peptide Ligands by the Parasitic Nematode Globodera rostochiensis
Mechanisms of Molecular Mimicry of Plant CLE Peptide Ligands by the Parasitic Nematode Globodera rostochiensis
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DOI：
10.1104/pp.111.180554
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发表时间：
2011-09-01
期刊：
影响因子：
7.4
通讯作者：
Clark, Steven E.
中科院分区：
文献类型：
--
作者：
Guo, Yongfeng;Ni, Jun;Clark, Steven E.
寄生在植物根系上的线虫造成巨大的经济损失，并且缺乏有效的防治机制。许多寄生线虫通过重新编程根系发育来感染植物，从而驱动取食结构的形成。线虫如何控制植物发育在很大程度上是未知的。在这里，我们确定了两个宿主因子参与受体配体模拟物GrCLE1的功能，GrCLE1是由马铃薯囊肿线虫Globodera rostochiensis分泌的。GrCLE1被宿主植物蛋白酶正确地加工成活性形式。经过加工的GrCLE1肽直接与植物CLE受体CLV2、BAM1和BAM2结合。在拟南芥中，GrCLE1肽改变植物根系发育的能力依赖于这些受体，这一事实也支持了这些受体参与配体模拟过程。重要的是，我们还证明了GrCLE1的成熟可以完全由植物因子进行，并且CLE加工活性的可用性可能是成功模仿配体的必要条件。
Nematodes that parasitize plant roots cause huge economic losses and have few mechanisms for control. Many parasitic nematodes infect plants by reprogramming root development to drive the formation of feeding structures. How nematodes take control of plant development is largely unknown. Here, we identify two host factors involved in the function of a receptor ligand mimic, GrCLE1, secreted by the potato cyst nematode Globodera rostochiensis. GrCLE1 is correctly processed to an active form by host plant proteases. Processed GrCLE1 peptides bind directly to the plant CLE receptors CLV2, BAM1, and BAM2. Involvement of these receptors in the ligand-mimicking process is also supported by the fact that the ability of GrCLE1 peptides to alter plant root development in Arabidopsis (Arabidopsis thaliana) is dependent on these receptors. Critically, we also demonstrate that GrCLE1 maturation can be entirely carried out by plant factors and that the availability of CLE processing activity may be essential for successful ligand mimicry.