Photovoltaic enzymes by design and evolution
Photovoltaic enzymes by design and evolution
复制标题
光伏酶的设计和进化
DOI：
10.1101/2022.12.20.521207
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Bunzel H
中科院分区：
文献类型：
--
作者：
Bunzel H
全球能源危机要求我们制定更有效的可持续能源生产战略。鉴于天然光合作用装置的卓越效率，生物混合光伏器件为太阳能转换提供了一种有吸引力的解决方案。然而，它们的组成、稳定性和复杂性限制了它们在光伏器件中的应用。在这里，我们结合计算设计和定向进化来克服这些限制并创建定制的光酶。通过将光敏剂结合位点引入含血红素的螺旋束蛋白中来设计光生物催化剂。设计的结合位点对目标光敏剂具有特异性，并且很容易移植到其他螺旋束中。最好的设计是高度可进化的，并在诱变和筛选后达到纳摩尔配体亲和力。进化后的酶产生的光电流比单独的光敏剂高 2.6 倍，这主要是由于光稳定性的提高。进化性是我们基于蛋白质的方法相对于非生物光伏的独特优势，对于开发高效的生物混合系统至关重要。
The global energy crisis challenges us to develop more efficient strategies for the sustainable production of energy. Given the excellent efficiency of the natural photosynthetic apparatus, biohybrid photovoltaic devices present an attractive solution for solar energy conversion. However, their composition, stability, and complexity can limit their inclusion into photovoltaic devices. Here, we combined computational design and directed evolution to overcome these limitations and create tailor-made photoenzymes. Photo-biocatalysts were designed by introducing photosensitizer binding sites into heme-containing helical bundle proteins. The designed binding sites were specific for the target photosensitizer and readily transplanted into other helical bundles. The best design was highly evolvable and reached nanomolar ligand affinity after mutagenesis and screening. The evolved enzyme generated 2.6 times higher photocurrents than the photosensitizer alone, primarily driven by increased photostability. Evolvability is a unique advantage of our protein-based approach over abiological photovoltaic and will be critical to developing efficient biohybrid systems.