Dispersive liquid-liquid microextraction based on a supramolecular solvent followed by high performance liquid chromatographic analysis of lignans in Forsythiae Fructus.
Dispersive liquid-liquid microextraction based on a supramolecular solvent followed by high performance liquid chromatographic analysis of lignans in Forsythiae Fructus.
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DOI：
10.1002/jssc.202200719
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发表时间：
2023-01
影响因子：
3.1
通讯作者：
Yu Qin;Runqin Wang;Rongrong Xing;Li Yang;Xuan Chen;Shuang-xi Hu
中科院分区：
文献类型：
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作者：
Yu Qin;Runqin Wang;Rongrong Xing;Li Yang;Xuan Chen;Shuang-xi Hu
提出了一种基于超分子溶剂的分散液液微萃取技术，结合高效液相色谱法对连翘中木脂素的提取和测定。将由四丁基溴化铵和正己醇组成的超分子溶剂与样品溶液混合，通过涡旋萃取分析物。完成提取后，通过离心分离提取相并收集用于HPLC分析。在这项工作中，优化了重要的萃取变量，例如萃取溶剂的类型和用量、样品相中的 pH 值和盐含量以及萃取时间。研究了超分子溶剂的合成，并通过透射电子显微镜对其微观结构进行了表征。在最佳条件下，所提议程序的分析物富集因子在 6 至 170 之间。获得了令人满意的线性范围 (r ≥ 0.99)、检测限 (0.025-0.4 ng/mL)、精密度 (<9.2%) 和准确度（回收率：96.5%-104.8%）。该方法已成功应用于连翘中木脂素的预富集，操作简单快速，成本低廉，环境友好。本文受版权保护。版权所有。
A supramolecular solvent based dispersive liquid-liquid microextraction was proposed for extraction and determination of lignans in Forsythiae Fructus combined with HPLC. The supramolecular solvent, consisting of tetrabutylammonium bromide and n-hexanol, was mixed with the sample solution to extract the analytes by a vortex. After accomplishing the extraction, the extraction phase was separated by centrifugation and collected for HPLC analysis. In this work, the important extraction variables such as the type and amount of extraction solvent, pH and salt amount in sample phase, and extraction time were optimized. The synthesis of supramolecular solvent was studied and its microstructure was characterized by transmission electron microscopy. Under the optimal conditions, the analytes' enrichment factors were between 6 and 170 for the proposed procedure. Satisfactory linear ranges (r ≥ 0.99), detection limits (0.025-0.4 ng/mL), precisions (<9.2%), and accuracies (recoveries: 96.5%-104.8%) were obtained. The method has been successfully applied to the preconcentration of lignans in Forsythiae Fructus with simple and rapid operation, low cost, and environmental friendliness. This article is protected by copyright. All rights reserved.