IMMUNOLOGICAL IDENTITY OF UROKINASE AND OVARIAN CARCINOMA PLASMINOGEN ACTIVATOR RELEASED IN TISSUE-CULTURE
IMMUNOLOGICAL IDENTITY OF UROKINASE AND OVARIAN CARCINOMA PLASMINOGEN ACTIVATOR RELEASED IN TISSUE-CULTURE
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DOI：
10.1038/261595a0
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发表时间：
1976-01-01
期刊：
影响因子：
64.8
通讯作者：
HOLMBERG, L
中科院分区：
文献类型：
--
作者：
ASTEDT, B;HOLMBERG, L
有证据表明纤维蛋白溶解酶参与肿瘤的生长。人们早就知道，培养中的恶性肿瘤会溶解凝结的血浆基质1 -3。Davidson等4发现肺巨细胞癌富含纤溶酶原激活物，并在组织培养中产生。Rifkinet al.5报道了DNA或RNA病毒转化的哺乳动物成纤维细胞、化学或病毒诱导的乳腺癌以及培养中的人类恶性肿瘤大量释放纤维蛋白溶解酶。正常成纤维细胞和其他正常组织培养物释放很少或没有纤溶酶。Christman和Acs 6发现，无论是由致癌病毒还是化学试剂转化的肿瘤细胞，都会释放正常细胞所不释放的纤溶酶原激活剂。该活化剂被表征为分子量约为50，000的丝氨酸蛋白酶。纤溶活性的存在已在人类卵巢肿瘤7以及与此类肿瘤相关的腹水8中得到证实。我们已经研究了一种稳定的纤溶酶原激活酶，释放卵巢癌组织培养，我们在这里报告的表观身份纤溶酶原激活剂（TA）和激活剂产生的胎儿肾脏和目前在正常尿液中，即尿激酶。
THERE is evidence that fibrinolytic enzymes are involved in the growth of tumours. It has long been known that malignant tumours in culture lyse clotted plasma substrates1–3. Davidsonet al.4found a giant-cell carcinoma of the lung to be rich in plasminogen activator and to produce it in tissue culture. Rifkinet al.5reported an abundant release of fibrinolytic enzymes by mammalian fibroblasts transformed by DNA or RNA viruses, by chemically or virally induced mammary carcinomas and by human malignant tumours in culture. Normal fibroblasts and cultures of other normal tissues released little or no fibrinolytic enzymes. Christman and Acs6found neoplastic cells, whether transformed by oncogenic viruses or by chemical agents, to release a plasminogen activator not released by normal cells. This activator was characterised as a serine protease with a molecular weight of approximately 50,000. The presence of fibrinolytic activity has been demonstrated in human ovarian tumours7as well as in ascitic fluid associated with such tumours8. We have studied a stable plasminogen-activating enzyme, released by ovarian carcinoma in tissue culture, and we report here the apparent identity of this plasminogen activator (TA) and the activator produced by foetal kidney and present in normal urine—that is, urokinase.