Usefulness of a tissue optical clearing technique for forensic autopsy
Usefulness of a tissue optical clearing technique for forensic autopsy
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DOI：
10.1111/1556-4029.14995
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发表时间：
2022-01-28
影响因子：
1.6
通讯作者：
Kominato，Yoshihiko
中科院分区：
文献类型：
--
作者：
Takahashi，Yoichiro;Hayakawa，Akira;Kominato，Yoshihiko
法医病理学家必须在尸体解剖时调查致命的创伤或疾病。除了各种器官的大面积挫伤外，还需要寻找一些具有潜在致命影响的小特征。由于此类病变可能需要进行显微镜检查以进行详细评估，因此选择合适的解剖位置进行组织取样非常重要。为了实际筛选小病变，我们已经开发了一种组织光学透明（TOC）技术法医尸检。该技术涉及用无毒有机溶剂肉桂酸乙酯进行清洁，使切除的器官透明，而血管或含血血管保持不透明。使用这种技术，能够通过肉眼检查识别脊髓中的微小血管，从而可以正确选择组织取样的位置。成功进行了后续组织病理学评价，没有与TOC手术相关的明显伪影。此外，TOC和靶向CT血管造影术的组合允许对上级肠系膜动脉中的动脉闭塞性病变进行可行的检查，并且当与micro-CT扫描组合时，其可用于评价支架植入的冠状动脉管腔。到目前为止所获得的结果表明，TOC可以补充常规法医尸检程序时，需要详细评估的小病变。
Forensic pathologists are required to investigate lethal trauma or disease at autopsy. In addition to massive contusions of various organs, a number of small features with potentially fatal implications also need to be sought. Since such lesions may need microscopic examinations for detailed evaluation, it is important to select suitable anatomic locations for tissue sampling. For practical screening of small lesions, we have developed a tissue optical clearing (TOC) technique for forensic autopsy. The technique involves clearing with a non‐toxic organic solvent, ethyl cinnamate, which renders excised organs transparent, while hemorrhages or blood‐containing vessels remain opaque. Using this technique, tiny hemorrhages in the spinal cord were able to be identified by gross examination, allowing proper selection of locations for tissue sampling. Subsequent histopathological evaluation was successfully performed with no apparent artifacts related with the TOC procedure. In addition, a combination of TOC and targeted CT angiography allowed feasible examination of the arterial occlusive lesion in the superior mesenteric artery, and when combined with micro‐CT scanning it was useful for evaluating the lumen of the coronary artery with stent implantation. The results obtained so far indicated that TOC could complement routine forensic autopsy procedures when detailed evaluation of small lesions is required.