Layer V neurons in mouse cortex projecting to different targets have distinct physiological properties
Layer V neurons in mouse cortex projecting to different targets have distinct physiological properties
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DOI：
10.1152/jn.00397.2007
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发表时间：
2007-12-01
影响因子：
2.5
通讯作者：
Nelson, Sacha B.
中科院分区：
文献类型：
--
作者：
Hattox, Alexis M.;Nelson, Sacha B.
小鼠皮层V层神经元投射到不同的靶点具有不同的生理特性。J Neurophysiol 98：3330-3340，2007.首次发表于2007年9月26日; doi：10.1152/jn.00397.2007。第V层锥体神经元在解剖学和生理学上是异质的，并且投射到多个皮质内和皮质下靶。然而，由于大多数对第V层锥体神经元的生理学研究都是在未鉴定的细胞上进行的，我们对皮层下投射部位的解剖和生理特性的关系知之甚少。在这里，我们将联合收割机神经解剖束追踪与小鼠体感皮层的全细胞记录相结合，以测试具有相同投射靶点的神经元是否形成离散的亚群，以及它们是否具有定型的生理特性。我们的研究结果表明，皮质丘脑和三叉神经元是两个很大程度上不重叠的亚群，而胼胝体和皮质纹状体神经元广泛重叠。皮质丘脑和皮质三叉神经元的形态以及内在膜和放电特性不同于胼胝体和皮质纹状体神经元。此外，我们发现，每一类投射神经元表现出独特的恭维超极化和去极化后电位，进一步表明，皮层神经元与不同的皮层下目标是彼此不同的。
Layer V neurons in mouse cortex projecting to different targets have distinct physiological properties. J Neurophysiol 98: 3330-3340, 2007. First published September 26, 2007; doi:10.1152/jn.00397.2007. Layer V pyramidal neurons are anatomically and physiologically heterogeneous and project to multiple intracortical and subcortical targets. However, because most physiological studies of layer V pyramidal neurons have been carried out on unidentified cells, we know little about how anatomical and physiological properties relate to subcortical projection site. Here we combine neuroanatomical tract tracing with whole cell recordings in mouse somatosensory cortex to test whether neurons with the same projection target form discrete subpopulations and whether they have stereotyped physiological properties. Our findings indicate that corticothalamic and -trigeminal neurons are two largely nonoverlapping subpopulations, whereas callosal and corticostriatal neurons overlap extensively. The morphology as well as the intrinsic membrane and firing properties of corticothalamic and corticotrigeminal neurons differ from those of callosal and corticostriatal neurons. In addition, we find that each class of projection neuron exhibits a unique compliment of hyperpolarizing and depolarizing afterpotentials that further suggests that cortical neurons with different subcortical targets are distinct from one another.