Arabidopsis thaliana CENTRORADIALIS homologue (ATC) acts systemically to inhibit floral initiation in Arabidopsis
Arabidopsis thaliana CENTRORADIALIS homologue (ATC) acts systemically to inhibit floral initiation in Arabidopsis
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DOI：
10.1111/j.1365-313x.2012.05076.x
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发表时间：
2012-10-01
期刊：
影响因子：
7.2
通讯作者：
Yu, Tien-Shin
中科院分区：
文献类型：
--
作者：
Huang, Nien-Chen;Jane, Wann-Neng;Yu, Tien-Shin
花的起始是由系统性的花激活剂和抑制剂协调的。这种遥控系统可以整合环境线索来调节花的启动。最近，FLOWERLOCUS T（FT）被发现是一种成花素。然而，系统性成花抑制剂或抗成花素的身份仍有待阐明。在这里，我们表明，拟南芥中心辐射同源物（ATC），拟南芥FT同源物，可能会采取行动，在非细胞自主的方式来抑制花的起始。ATC无效突变体的分析表明，ATC是一个短日照诱导的成花抑制剂。细胞类型特异性表达表明，表达ATC的伴细胞和顶端足以抑制成花。组织化学分析表明，ATC的启动子活性主要存在于维管组织中，但在顶端的检测限以下，表明ATC可能从维管组织向顶端移动，从而影响开花。与这一观点相一致，拟南芥幼苗嫁接实验表明，ATC移动了很长的距离，ATC的成花抑制是嫁接传递的。ATC可能拮抗FT活性，因为ATC和FT都与FD相互作用，并以相反的方式影响相同的下游分生组织身份基因APETALA 1。因此，光周期的变化可能会触发功能相反的FT同系物系统地影响成花。
Floral initiation is orchestrated by systemic floral activators and inhibitors. This remote-control system may integrate environmental cues to modulate floral initiation. Recently, FLOWERING LOCUS T (FT) was found to be a florigen. However, the identity of systemic floral inhibitor or anti-florigen remains to be elucidated. Here we show that Arabidopsis thaliana CENTRORADIALIS homologue (ATC), an Arabidopsis FT homologue, may act in a non-cell autonomous manner to inhibit floral initiation. Analysis of the ATC null mutant revealed that ATC is a short-day-induced floral inhibitor. Cell type-specific expression showed that companion cells and apex that express ATC are sufficient to inhibit floral initiation. Histochemical analysis showed that the promoter activity of ATC was mainly found in vasculature but under the detection limit in apex, a finding that suggests that ATC may move from the vasculature to the apex to influence flowering. Consistent with this notion, Arabidopsis seedling grafting experiments demonstrated that ATC moved over a long distance and that floral inhibition by ATC is graft transmissible. ATC probably antagonizes FT activity, because both ATC and FT interact with FD and affect the same downstream meristem identity genes APETALA1, in an opposite manner. Thus, photoperiodic variations may trigger functionally opposite FT homologues to systemically influence floral initiation.