Interactions between Golli-MBP and Th1/Th2 cytokines in patients with oral lichen planus
Interactions between Golli-MBP and Th1/Th2 cytokines in patients with oral lichen planus
复制标题
口腔扁平苔藓患者 Golli-MBP 与 Th1/Th2 细胞因子之间的相互作用
DOI：
10.1111/odi.12090
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发表时间：
2014-03-01
期刊：
影响因子：
3.8
通讯作者：
Fan, Y.
中科院分区：
文献类型：
--
作者：
Ding, M.;Zeng, J.;Fan, Y.
口腔扁平苔藓（OLP）是一种常见的细胞介导的自身免疫性疾病，病因不明。Th 1/Th 2平衡的破坏已被怀疑有助于OLP的发病机制。在这项研究中，我们的目的是探索，在口腔扁平苔藓，Th 1/Th 2细胞因子和Golli-MBP，调节器的autogenic T cells.Subjects和Methods 36 OLP患者年龄18-79和19名对照组年龄20-69岁，在南京医科大学的牙科诊所参加了相互作用。OLP的临床诊断和组织病理学检查证实。所有受试者均无其他自身免疫性疾病。收集所有受试者的外周血单核细胞（PBMC）和血清。Golli-MBP，IFN-γ和IL-4的基因表达和蛋白水平分别测定，通过半定量逆转录聚合酶链反应和酶联免疫吸附试验。ResultsWhen与对照组相比，PBMC和血清从OLP患者表现出显着较高的表达和浓度Golli-MBP和IL-4和较低的IFN-γ水平。呈强负相关（r=-0.838，P
ObjectiveOral lichen planus (OLP) is a prevalent cell-mediated autoimmune disease of unknown etiology. Disruption in the Th1/Th2 equilibrium has been suspected to contribute to the pathogenesis of OLP. In this study, we aimed at exploring, in OLP, the interactions between Th1/Th2 cytokines and Golli-MBP, a regulator of autogenic T cells.Subjects and MethodsThirty-six OLP patients aged 18-79 and nineteen control subjects aged 20-69 were enrolled in the dental clinics of Nanjing Medical University. OLP was diagnosed clinically and verified by histopathological examination. All subjects were free of other autoimmune diseases. Peripheral blood mononuclear cells (PBMC) and serum were collected from all subjects. Gene expression and protein levels of Golli-MBP, IFN- and IL-4 were measured, respectively, by semiquantitative reverse transcription polymerase chain reaction and enzyme-linked immunosorbent assay.ResultWhen compared with control, PBMC and serum from OLP patients exhibited a significantly higher expression and concentration of Golli-MBP and IL-4 and lower levels of IFN-. A strong negative correlation (r=-0.838, P