Age-related change and its sex differences in histoarchitecture of the hypothalamic suprachiasmatic nucleus of F344/N rats
Age-related change and its sex differences in histoarchitecture of the hypothalamic suprachiasmatic nucleus of F344/N rats
复制标题
DOI：
10.1016/j.exger.2004.10.003
复制
发表时间：
2005-03-01
影响因子：
3.9
通讯作者：
Kitagawa, H
中科院分区：
文献类型：
--
作者：
Tsukahara, S;Tanaka, S;Kitagawa, H
本研究探讨了衰老和性别差异对 F344/N 大鼠视交叉上核 (SCN) 的影响。在幼年(1.6-1.9月龄)、成年(11.7-16.3月龄)和老年(29.2-34月龄)大鼠中，用甲酚固紫染色切片测定SCN的体积、神经元核大小和神经元细胞数。此外，还对胶质纤维酸性蛋白（GFAP）进行了免疫组织化学分析。年龄和性别差异对 SCN 体积没有显着影响。雌性从幼年到老年，视交叉上核的神经元数量逐渐减少。然而，在雄性大鼠中，成年和老年大鼠的数量显着减少。除了男性视交叉上核的腹外侧部分外，两性的视交叉上核中神经元核的大小均随着年龄的增长而显着减小。在雌性SCN背内侧部分，成年大鼠的GFAP免疫反应成分的密度显着高于其他年龄的大鼠。然而，成年男性的 SCN 密度没有显着增加。这些结果表明，随着年龄的增长，视交叉上核中神经元和星形胶质细胞的形态变化以性别特异性的方式发生。 (c) 2004 Elsevier Inc. 保留所有权利。
The present study examined the effects of aging and sex differences on the suprachiasmatic nucleus (SCN) of F344/N rats. In juveniles (1.6-1.9 months of age), adults (11.7-16.3 months of age), and old (29.2-34 months of age) rats, the volume, size of neuronal nucleus and neuronal cell number of the SCN were determined with cresyl fast violet-stained sections. In addition, immunohistochemical analysis was performed for glial fibrillary acidic protein (GFAP). There was no significant effect of aging and sex differences on the SCN volume. The number of neurons in the SCN gradually decreased from juvenile to old age in females. However, in males, the number was significantly decreased in adult and old age rats. The size of neuronal nuclei in the SCN was significantly decreased by increasing age in both sexes, except for the ventrolateral part of the SCN of males. In the dorsomedial part of the SCN of females, the density of GFAP-immunoreactive components was significantly higher in adult age rats than in rats of other ages. However, there was no significant increase in the density of the SCN in adult males. These results suggest that morphological changes in neuronal and astroglial cells occur in the SCN with aging in a sex-specific manner. (c) 2004 Elsevier Inc. All rights reserved.