High-performance liquid chromatography/tandem mass spectrometry assay for the rapid high sensitivity measurement of basal acetylcholine from microdialysates
High-performance liquid chromatography/tandem mass spectrometry assay for the rapid high sensitivity measurement of basal acetylcholine from microdialysates
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DOI：
10.1016/s0165-0270(02)00228-5
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发表时间：
2002-11-15
影响因子：
3
通讯作者：
Shah, AJ
中科院分区：
文献类型：
--
作者：
Hows, MEP;Organ, AJ;Shah, AJ
建立了一种高通量液相色谱-串联质谱（LC/MS/MS）分析脑透析液中乙酰胆碱（ACh）的方法。乙酰胆碱的这种分离是基于阳离子交换色谱法，洗脱缓冲液由乙酸铵、甲酸铵和乙腈的混合物组成。使用等度分离条件，乙酰胆碱在一分钟内解决，并使用串联质谱法在正离子电喷雾模式检测。ACh的检测限为1 fmol，S/N比为3：1。该测定已被常规用于测量清醒自由活动大鼠脑透析液中的乙酰胆碱。此外，分离条件进行了修改，允许同时测量乙酰胆碱和乙酰胆碱酯酶抑制剂，新斯的明。(C)2002 Elsevier Science B. V.保留所有权利。
A high-throughput liquid chromatography tandem mass spectrometry (LC/MS/MS) method has been developed for the analysis of acetylcholine (ACh) in brain dialysates. This separation of ACh is based on cation exchange chromatography with elution buffer consisting of a mixture of ammonium acetate, ammonium formate and acetonitrile. Using isocratic separation conditions, ACh was resolved within a minute and detected using tandem mass spectrometry in the positive ion electrospray mode. The limit of detection for ACh was found to be 1 fmol on column with a S/N ratio of 3:1. The assay has been used routinely for the measurement of ACh in brain dialysates from awake freely moving rats. Furthermore, separation conditions were modified to allow simultaneous measurement of ACh and the acetylcholine esterase inhibitor, neostigmine. (C) 2002 Elsevier Science B.V. All rights reserved.