Osteoclasts in the Dental Microenvironment: A Delicate Balance Controls Dental Histogenesis
Osteoclasts in the Dental Microenvironment: A Delicate Balance Controls Dental Histogenesis
复制标题
DOI：
10.1159/000324787
复制
发表时间：
2011-01-01
影响因子：
2.7
通讯作者：
Lezot, F.
中科院分区：
文献类型：
--
作者：
Berdal, A.;Castaneda, B.;Lezot, F.
破骨细胞活性对牙齿发育的影响以前已经分析过，但在严重的骨硬化症的背景下。本研究旨在研究Msx 2基因敲入突变小鼠（Msx 2-/-）中存在的破骨细胞功能低下和驱动破骨细胞前体RANK过度表达的转基因小鼠（RANK（Tg））中的破骨细胞功能亢进对牙齿发育的影响。在Msx 2-/-小鼠中，观察到中度骨硬化症，仅发生在牙周区域。显微放射学和组织学分析显示，异常的牙上皮组织发生，引起牙源性肿瘤样结构。这导致牙齿萌出受损，尤其是下颌第三磨牙。在RANK（Tg）小鼠中，根组织发生显示牙细胞增殖和分化率的位点特异性上调。这最终导致根的直径和纸浆大小减小，尽管长度正常。这两个反向实验系统将使不同的牙齿细胞和破骨细胞的通信在正常生长和肿瘤发生的调查。版权所有（C）2011 S. Karger AG，巴塞尔
The impact of osteoclast activity on dental development has been previously analyzed but in the context of severe osteopetrosis. The present study sought to investigate the effects of osteoclast hypofunction, present in Msx2 gene knockin mutant mice (Msx2-/-), and hyperfunction, in transgenic mice driving RANK over-expression in osteoclast precursors (RANK(Tg)), on tooth development. In Msx2-/- mice, moderate osteopetrosis was observed, occurring exclusively in the periodontal region. Microradiographical and histological analyses revealed an abnormal dental epithelium histogenesis that gave rise to odontogenic tumor-like structures. This led to impaired tooth eruption, especially of the third mandibular molars. In RANK(Tg) mice, root histogenesis showed site-specific upregulation of dental cell proliferation and differentiation rates. This culminated in roots with a reduced diameter and pulp size albeit of normal length. These two reverse experimental systems will enable the investigation of distinctive dental cell and osteoclast communication in normal growth and tumorigenesis. Copyright (C) 2011 S. Karger AG, Basel