Platelet polyphosphate and energy metabolism in professional male athletes (soccer players): A cross-sectional pilot study.
Platelet polyphosphate and energy metabolism in professional male athletes (soccer players): A cross-sectional pilot study.
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DOI：
10.14814/phy2.15409
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发表时间：
2022-08
影响因子：
2.5
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人血小板聚磷酸(Polyp)是一种多功能分子，但其功能尚不完全清楚。最近的一项研究表明，与骨骼肌相似，息肉也参与了血小板的能量代谢，这表明训练有素的运动员可能会表现出较高的血小板息肉水平来储存能量。为了验证这一假设，我们量化了血小板息肉和NADH，NADH是同一代未受过训练和受过良好训练的日本男性参与者中参与ATP产生的一种成分。从年轻、健康、非运动员和职业足球运动员(职业运动员)的静脉血中制备洗涤过的血小板。NADH和息肉的测定采用四唑还原分光光度法，4‘，6-二氨基-2-苯基吲哚荧光法，激发/发射波长为425/525 nm。同时测量体重和阻抗。采用Mann-Whitney U检验和Spearman相关系数进行统计分析。尽管基础代谢率水平显著高于非运动员组，但职业运动员组的血小板息肉水平显著低于非运动员组。其他身体成分和血小板指标两组间均无显著差异。亲运动员组的血小板息肉和NADH水平之间显示出中等的、接近显著的相关性(R= 0.439；P= 0.0512)。结合息肉与体重指数的弱相关性数据，提示血小板息肉水平可能受血小板和机体能量代谢活动的影响。需要进一步的生化研究来阐明这一机制。虽然多聚磷酸(Polyp)最近已被认为是能量代谢的代谢物，但其在人类血小板中的代谢和功能仍未得到很好的研究。可以假设，训练有素的专业运动员通常比非运动员表现出更高的基础代谢率，他们也可能表现出用于储存能量的高水平的血小板息肉。这项横断面研究强调，与我们的假设相反，优秀运动员的血小板息肉水平显著低于非运动员。
Human platelet polyphosphate (polyP) is a multifunctional molecule; however, its functions are not yet fully understood. A recent study demonstrated that similar to skeletal muscle, polyP is involved in energy metabolism in platelets, which suggests that well‐trained athletes may exhibit elevated platelet polyP levels for energy storage. To test this hypothesis, we quantified platelet polyP along with NADH, a component involved in ATP production in non‐trained and well‐trained male Japanese participants of the same generation. Washed platelets were prepared from the venous blood of young, healthy, non‐athletes, and professional soccer players (pro‐athletes). NADH and polyP levels were spectrophotometrically determined using tetrazolium reduction and fluorometrically determined using 4′,6‐diamidino‐2‐phenylindole at the excitation/emission wavelengths of 425/525 nm. Body weight and impedances were measured simultaneously. Statistical analyses were performed using the Mann–Whitney U test and Spearman correlation coefficient. Although basal metabolic rate levels were significantly higher, platelet polyP levels were significantly lower in pro‐athletes than in that in non‐athletes. No significant differences were detected in other body compositions or platelet indices between the two groups. The pro‐athlete group showed a moderate, nearly significant correlation (R = 0.439; p = 0.0512) between platelet polyP and NADH levels. Taken together with the weak correlation data between polyP and body mass index, it is suggested that platelet polyP levels may be influenced by platelet and body energy metabolic activity. Further biochemical studies are needed to elucidate this mechanism. Although polyphosphate (polyP) has recently been accepted as a metabolite of energy metabolism, its metabolism and function remain poorly explored in human platelets. It could be hypothesized that well‐trained professional athletes who generally exhibit higher basal metabolic rate than non‐athletes may also exhibit elevated levels of platelet polyP for energy storage. This cross‐sectional study highlights that against our hypothesis, platelet polyP levels are significantly lower in elite athletes than in non‐athletes.