Expression of multiple gamma-glutamyl transpeptidase messenger ribonucleic acid transcripts in the adult rat epididymis is differentially regulated by androgens and testicular factors in a region-specific manner.
Expression of multiple gamma-glutamyl transpeptidase messenger ribonucleic acid transcripts in the adult rat epididymis is differentially regulated by androgens and testicular factors in a region-specific manner.
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DOI：
10.1210/endo.135.3.7915228
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发表时间：
1994-09
期刊：
影响因子：
4.8
通讯作者：
M. A. Palladino;B T Hinton
中科院分区：
文献类型：
--
作者：
M. A. Palladino;B T Hinton
多种γ-谷氨酰转肽酶（GGT）信使RNA（mRNAs Ⅱ-Ⅳ）在大鼠附睾中以区域特异性方式表达。在本研究中，我们检测了血浆睾酮（T）和睾丸因子在调节附睾管沿着表达的GGT mRNA的区域特异性模式和数量中的作用。北方印迹和核糖核酸酶保护分析表明，双侧睾丸切除1，5和15天显着降低GGT mRNAs Ⅱ-Ⅳ在初始段的表达。GGT mRNAII和mRNAIII的表达在接受T植入物的睾丸切除动物的初始段中保持，所述T植入物维持正常血清T浓度，但GGT mRNAIV表达相对于假手术对照值保持较低。单侧输出管结扎仅在起始段降低GGT mRNAIV表达。因此，GGT mRNAIV在起始节段的表达不受循环T的维持，需要睾丸来源的因子。在附睾头，睾丸切除术后GGT mRNA II和mRNA III的表达下降，并且仅通过血浆T不能完全恢复到睾丸切除动物的对照值，但也需要睾丸因子。与初始片段相反，在附睾体和尾部中GGT mRNA IV的表达不需要T和/或睾丸因子，因为在睾丸切除术、睾丸切除术和T替代术后1、5和15天以及单侧输出管结扎后，该转录物在这些区域中的表达保持不变。在附睾尾，GGT mRNA II的表达需要循环雄激素，不受单侧输出管结扎的影响，而GGT mRNA III的表达被T.这些数据表明，循环T和睾丸来源的因子以区域特异性方式差异调节每个GGT mRNA的表达。
Multiple gamma-glutamyl transpeptidase (GGT) messenger RNAs (mRNAsII-IV) are expressed in a region-specific manner in the rat epididymis. In the present study, we examined the role(s) of plasma testosterone (T) and testicular factors in regulating the region-specific pattern and quantity of GGT mRNAs expressed along the epididymal duct. Northern blot and ribonuclease protection analyses showed that bilateral orchiectomy for 1, 5, and 15 days dramatically reduced the expression of GGT mRNAsII-IV in the initial segment. Expression of GGT mRNAII and mRNAIII was maintained in the initial segment of orchiectomized animals receiving T implants that maintain normal serum T concentrations, but GGT mRNAIV expression remained low relative to sham-operated control values. Unilateral efferent duct ligation decreased GGT mRNAIV expression only in the initial segment. Hence, expression of GGT mRNAIV in the initial segment was not maintained by circulating T and required a factor(s) of testicular origin. In caput epididymidis, expression of GGT mRNAII and mRNAIII declined after orchiectomy and was not completely restored to control values in orchiectomized animals by plasma T alone, but also required a testicular factor(s). In contrast to the initial segment, expression of GGT mRNAIV in the corpus and cauda epididymidis did not require T and/or a testicular factor(s), as expression of this transcript remained unchanged in these regions after 1, 5, and 15 days of orchiectomy, orchiectomy and T replacement, and after unilateral efferent duct ligation. In the cauda epididymidis, expression of GGT mRNAII required circulating androgens and was unaffected by unilateral efferent duct ligation, whereas GGT mRNAIII expression was repressed by T. These data demonstrate that circulating T and a factor(s) of testicular origin differentially regulate the expression of each GGT mRNA in a region-specific manner.