Isolation of five Rubrobacter strains from biodeteriorated monuments
Isolation of five Rubrobacter strains from biodeteriorated monuments
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DOI：
10.1007/s00114-008-0452-2
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发表时间：
2009-01-01
影响因子：
--
通讯作者：
Saiz-Jimenez, C.
中科院分区：
文献类型：
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作者：
Laiz, L.;Miller, A. Z.;Saiz-Jimenez, C.
在过去的几年里，微生物在古代纪念碑壁画上的定植已经引起了微生物学家和保护学家的注意。红杆菌属常见于生物变质的纪念碑中，据报道它会引起玫瑰色变色。然而，到目前为止，这个属只有三种被分离出来，都来自嗜热环境。在本文中，我们研究了三个纪念碑：Servilia和Postumio的罗马墓地Carmona（西班牙）和Vilar de Frades教堂（葡萄牙），以寻找Rubrobacter菌株。在所有情况下，观察到生物变质和开花的形成，并分离到5株鲁布罗杆菌。在变性梯度凝胶电泳中，这些分离株表现出不同的生理和迁移特征，表明它们可能是本属的新种。分离株在实验室中再现了一些生物变质过程，揭示了它们在晶体形成中的生物中介作用。
In the last few years, the microbial colonisation of mural paintings in ancient monuments has been attracting the attention of microbiologists and conservators. The genus Rubrobacter is commonly found in biodeteriorated monuments, where it has been reported to cause rosy discolouration. However, to date, only three species of this genus have been isolated, all from thermophilic environments. In this paper, we studied three monuments: the Servilia and Postumio tombs in the Roman Necropolis of Carmona (Spain), and Vilar de Frades church (Portugal), in search of Rubrobacter strains. In all cases, biodeterioration and the formation of efflorescences were observed, and five Rubrobacter strains were isolated. These isolates showed different physiology and migration in denaturing gradient gel electrophoresis, suggesting they might represent new species within this genus. The isolates reproduced some biodeterioration processes in the laboratory and revealed their biomediation in crystal formation.