Overexpression of the Wilms' tumor gene WT1 in colorectal adenocarcinoma
Overexpression of the Wilms' tumor gene WT1 in colorectal adenocarcinoma
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DOI：
10.1111/j.1349-7006.2003.tb01507.x
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发表时间：
2003-08-01
期刊：
影响因子：
5.7
通讯作者：
Sugiyama, H
中科院分区：
文献类型：
--
作者：
Oji, Y;Yamamoto, H;Sugiyama, H
检测59例大肠腺癌中Wilms肿瘤基因WT 1的表达，以探讨WT 1在肿瘤发生中的作用。实时荧光定量RT-PCR结果显示，WT 1 mRNA的表达量为7.2 × 10 ~（-5）~ 4.9 × 10 ~（-1（K562白血病细胞中WT 1表达水平定义为1.0）在所有（100%）的28例结直肠腺癌中，28例中有20例（71%）的WT 1 mRNA表达水平高于正常黏膜组织。采用抗WT 1抗体对46例结直肠腺癌（28例WT 1 mRNA表达的15例和31例新收集的病例）进行免疫组化分析，46例中有41例（89%）检测到WT 1蛋白表达。WT 1基因组DNA的直接测序分析显示，在5种不同的结直肠腺癌中，WT 1基因的10个外显子中没有任何突变。这些结果可能表明野生型WT 1基因在大肠腺癌的发生中起重要作用。
Expression of the Wilms' tumor gene WT1 was examined in 59 cases of colorectal adenocarcinoma to examine the involvement of WT1 in tumorigenesis. Quantitative real-time reverse transcription-polymerase chain reaction (RT-PCR) showed that WT1 mRNA was expressed in the range from 7.2x10(-5) to 4.9x10(-1) levels (WT1 expression level in K562 leukemic cells was defined as 1.0) in all (100%) of the 28 cases of colorectal adenocarcinoma examined, and that the WT1 mRNA expression levels were higher in 20 (71%) of the 28 cases compared to those of normal-appearing mucosal tissues examined. Immunohistochemical analysis using an anti-WT1 antibody was performed on 46 cases of colorectal adenocarcinoma (15 of the 28 cases with WT1 mRNA expression and 31 newly collected cases), and the expression of WT1 protein was detected in 41 (89%) of the 46 cases. The direct sequencing analysis of the WT1 genomic DNA showed no mutations in any of the 10 exons of the WT1 gene in any of 5 different colorectal adenocarcinomas. These results may indicate an important role of the wild-type WT1 gene in tumorigenesis of colorectal adenocarcinoma.