Biosynthesis of Territrems by Aspergillus terreus
Biosynthesis of Territrems by Aspergillus terreus
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土曲霉 (Aspergillus terreus) 生物合成 Territrems
DOI：
10.1128/aem.54.2.585-587.1988
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发表时间：
1988
影响因子：
4.4
通讯作者：
Y. Peng
中科院分区：
文献类型：
--
作者：
K. Ling;Y. Peng
将不同的放射性前体添加到土曲霉 23-1 的 8 天马铃薯葡萄糖液体培养物中。第14天，从培养物的氯仿提取物中分离土鳖，并通过薄层色谱法和高压液相色谱法纯化。将得到的territrem B用碱性过氧化氢处理，从反应混合物的乙酸乙酯萃取液中分离出3,4,5-三甲氧基苯甲酸，并通过薄层色谱法和高压液相色谱法进行纯化。通过比较territrem B及其芳香裂解产物的比放射性（每微摩尔化合物每分钟崩解数），证明当[U-14C]莽草酸、l-[甲基-14C]蛋氨酸和l-[甲基-3H]蛋氨酸为前体时，territrem B的放射性主要位于其芳香部分；然而，当[2-14C]甲羟戊酸为前体时，territrem B 的放射性主要位于其非芳香族部分。 Mevinolin 是一种 β-羟基 β-甲基戊二酰辅酶 A 还原酶的特异性抑制剂，可抑制 A. terreus 23-1 产生 territrems。当使用[U-14C]乙酸盐作为前体时，mevinolin抑制放射性碳并入territrem，但mevinolin不抑制来自[2-14C]甲羟戊酸盐的放射性碳并入territrem。
Different radioactive precursors were added to 8-day potato-dextrose liquid cultures of Aspergillus terreus 23-1. Territrems were isolated from chloroform extracts of the cultures at day 14 and purified by thin-layer chromatography and high-pressure liquid chromatography. The territrem B obtained was treated with alkaline hydrogen peroxide, and 3, 4, 5-trimethoxy benzoic acid was isolated from an ethyl acetate extract of the reaction mixture and purified by thin-layer chromatography and high-pressure liquid chromatography. By comparison of the specific radioactivities of territrem B and its cleaved aromatic product (disintegrations per minute per micromole of compound), it was demonstrated that the radioactivity of territrem B was located mainly on its aromatic moiety when [U-14C]shikimate, l-[methyl-14C]methionine, and l-[methyl-3H]methionine were precursors; however, the radioactivity of territrem B was located mainly on its nonaromatic moiety when [2-14C]mevalonate was the precursor. Mevinolin, a specific inhibitor of β-hydroxyl β-methyl glutaryl coenzyme A reductase, was shown to inhibit production of territrems by A. terreus 23-1. When [U-14C]acetate was used as a precursor, mevinolin inhibited the incorporation of radioactive carbon into territrem but mevinolin did not inhibit incorporation of radioactive carbon from [2-14C]mevalonate into territrem.