Relative biological effectiveness for cell-killing effect on various human cell lines irradiated with heavy-ion medical accelerator in CHIBA (HIMAC) carbon-ion beams
Relative biological effectiveness for cell-killing effect on various human cell lines irradiated with heavy-ion medical accelerator in CHIBA (HIMAC) carbon-ion beams
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DOI：
10.1016/s0360-3016(00)00568-x
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发表时间：
2000-08-01
影响因子：
7
通讯作者：
Ando, K
中科院分区：
文献类型：
--
作者：
Suzuki, M;Kase, Y;Ando, K
目的：为了明确2种不同线性能量转移（LET）束流的碳离子束对不同人体细胞系的相对生物学效应（RBE）值，并研究定性不同种类的辐射对细胞杀伤效应与生物物理特性（如染色体数目和细胞核面积）之间的关系。16种不同的人类细胞系用碳离子束照射，具有2种不同的LET值（LET_（max）= 13.3和约77 keV/μ m），由日本国立放射科学研究所的千叶重离子医用加速器（HIMAC）加速。使用集落形成测定法检测细胞杀伤作用作为生殖细胞死亡。使用常规方法观察中期涂片中的染色体数量，使用千分尺在照片上计算细胞核面积为椭圆，结果：通过D-10计算的RBE值，其被确定为相对于X射线将存活分数降低至10%所需的剂量（戈伊），13 keV/μ m束辐照各细胞系的D-10值在1.06 ~ 1.33之间，约77 keV/μ m束辐照各细胞系的D-10值在2.00 ~ 3.01之间。然而，D-10值并没有明显地依赖于染色体数目或细胞核的面积。结论：HIMAC碳离子束的RBE值是一致的，与以前的报告使用的碳离子束具有类似的LET值，和不同细胞系的细胞辐射敏感性，以及不同类型的辐射之间的相关性。(C)2000 Elsevier Science Inc.
Purpose: To clarify the relative biological effectiveness (RBE) values of various human cell lines for carbon-ion beams with 2 different linear energy transfer (LET) beams and to investigate the relationship between the cell-killing effect and the biophysical characters, such as the chromosome number and the area of the cell nucleus, using qualitatively different kinds of radiations.Methods and Materials: Sixteen different human cell lines were irradiated with carbon-ion beams, having 2 different LET values (LETinfinity = 13.3 and approximately 77 keV/mu m), accelerated by the Heavy Ion Medical Accelerator in Chiba (HIMAC) at National Institute of Radiological Sciences in Japan. Cell-killing effect was detected as reproductive cell death using a colony-formation assay. The number of chromosomes was observed in a metaphase spread using the conventional method, The area of the cell nucleus was calculated as an ellipse on photographs using a micrometer,Results: The RBE values calculated by the D-10, which is determined as the dose (Gy) required to reduce the surviving fraction to 10%, relative to X-rays, range from 1.06 to 1.33 for 13-keV/mu m-beam and from 2.00 to 3.01 for approximate 77-keV/mu m-beam irradiation on each cell line, There was a good correlation in the D-10 values of each cell line between X-rays and carbon-ion beams. However, the D-10 values did not clearly depend on either the chromosome number or the area of the cell nuclei.Conclusion: The RBE values for HIMAC carbon-ion beams are consistent with previous reports using carbon-ion beams with the similar LET values, and the cellular radiosensitivity of different cell lines,well correlate among different types of radiation. (C) 2000 Elsevier Science Inc.