Translation of synonymous codons in family boxes by Mycoplasma capricolum tRNAs with unmodified uridine or adenosine at the first anticodon position.
Translation of synonymous codons in family boxes by Mycoplasma capricolum tRNAs with unmodified uridine or adenosine at the first anticodon position.
复制标题
通过在第一个反密码子位置具有未修饰的尿苷或腺苷的山羊支原体 tRNA 翻译家族盒中的同义密码子。
DOI：
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发表时间：
1995
影响因子：
5.6
通讯作者：
S. Osawa
中科院分区：
文献类型：
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作者：
Y. Inagaki;Atsushi Kojima;Y. Bessho;H. Hori;T. Ohama;S. Osawa
在山羊肌浆菌中，除了精氨酸和苏氨酸（CUN亮氨酸、GUN缬氨酸、UCN丝氨酸、CCN脯氨酸、GCN丙氨酸和GGN甘氨酸）之外的密码子家族盒仅具有单个tRNA种类，其具有反密码子序列UNN（N是U、C、A或G），第一个核苷U未修饰。用M. capriculum无细胞翻译系统。对在编码框中含有每个相应氨基酸密码子的合成mRNA进行翻译。tRNAUNN翻译所有家族盒密码子的效率相似，如果不是相等的话。In M.在山羊中，有两种苏氨酸tRNA，tRNA（UGUThr）和tRNA（AGUThr），第一个核苷U或A是未修饰的。tRNA（UGUThr）种类有效地翻译密码子ACA、ACG和ACU，而ACC仅差地翻译。相比之下，tRNA（AGUThr）种类有效地翻译密码子ACU、ACC和ACG，而ACA较差。
In Myocoplasma capricolum, codon family boxes except for arginine and threonine (CUN leucine, GUN valine, UCN serine, CCN proline, GCN alanine, and GGN glycine) have only a single tRNA species with the anticodon sequence UNN (N is U, C, A or G), the first nucleoside U being unmodified. Incorporation of the [3H]amino acid into the peptide fraction was examined with the M. capricolum cell-free translation system. Synthetic mRNA containing each of the respective amino acid codons in the coding frame was subjected to translation. The tRNAUNN translated all the family box codons with similar, if not equal, efficiencies. In M. capricolum, there are two species of threonine tRNA, tRNA(UGUThr) and tRNA(AGUThr), the first nucleoside U or A being unmodified. The tRNA(UGUThr) species translates codons ACA, ACG and ACU efficiently, and ACC only poorly. In contrast, the tRNA(AGUThr) species translates codons ACU, ACC and ACG efficiently and ACA poorly.