Synthesis and reaction of DNA oligomers containing modified cytosines related to bisulfite sequencing.
Synthesis and reaction of DNA oligomers containing modified cytosines related to bisulfite sequencing.
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与亚硫酸氢盐测序相关的含有修饰胞嘧啶的 DNA 寡聚物的合成和反应。
DOI：
10.1021/ol900195z
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发表时间：
2009
期刊：
影响因子：
5.2
通讯作者：
K. Nakatani
中科院分区：
文献类型：
--
作者：
Y. Oka;T. Peng;F. Takei;K. Nakatani
本文报道了用配体诱导胞嘧啶碱基翻转，然后同时加入亚硫酸氢盐和羟胺合成含N（4）-羟基-5，6-二氢胞嘧啶-6-磺酸盐的DNA寡聚体。与C相反，翻转的5-甲基胞嘧啶被高锰酸钾选择性地氧化超过双链体中的胸腺嘧啶和胞嘧啶。配体诱导的碱基翻转是合成修饰胞嘧啶和5-甲基胞嘧啶的一种方便而有效的策略，与亚硫酸氢盐测序有关。
The synthesis of DNA oligomers containing N(4)-hydroxy-5,6-dihydrocytosine-6-sulfonate by using ligand-induced base flipping of cytosine followed by the simultaneous addition of bisulfite and hydroxylamine is reported. In contrast to C, the flipped-out 5-methylcytosine was selectively oxidized over thymines and cytosines in the duplex by potassium permanganate. Ligand-induced base flipping is a convenient and powerful strategy for the synthesis of modified cytosines and 5-methylcytosines related to bisulfite sequencing at the predetermined site of DNA.