Characterization of adipose-derived stem cells of anatomical region from mice.
Characterization of adipose-derived stem cells of anatomical region from mice.
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DOI：
10.1186/1756-0500-7-552
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发表时间：
2014-08-20
影响因子：
1.8
通讯作者：
Maria DA
中科院分区：
文献类型：
--
作者：
Luna AC;Madeira ME;Conceição TO;Moreira JA;Laiso RA;Maria DA
干细胞是一组具有自我更新、长期生存能力和多谱系潜能的细胞。几项研究提供了证据表明，脂肪组织代表了干细胞的替代来源，脂肪来源的干细胞的主要好处是，它们可以通过简单的微创方法从患者身上轻松收获，并且可以轻松培养。本研究的目的是建立一种从C57 BL/6 J小鼠获得脂肪干细胞（ADSCs）并鉴定其特性的培养方法。结果表明，从卵巢和附睾分离的脂肪组织的解剖区域的年龄不同，产量，活力和细胞形态不同。细胞周期蛋白D1的测定结果表明，不同群体的脂肪干细胞之间的表达一致。120天后，在小鼠的大细胞群中也观察到胱天蛋白酶-3活性表达的显著增加。ADSC对间充质标志物CD 90和CD 105、Nanog、SSEA-1、CD 106和VEGFR-1呈阳性，对造血标志物CD 34和CD 45呈阴性。在两种性别中也观察到大量处于S + G2/M期的细胞，表明ADSC的高增殖能力。我们观察到，从所研究的解剖区域分离的C57 BL/6 J小鼠的脂肪组织是获得具有高活力和增殖反应的多能间充质干细胞的有希望的来源。
Stem cells constitute a group of great capacity for self-renewal, long-term viability, and multi-lineage potential. Several studies have provided evidence that adipose tissue represents an alternative source of stem cells, with the main benefit of adipose-derived stem cells being that they can be easily harvested from patients by a simple minimally invasive method and can be easily cultured. The aim of this study was to establish a culture protocol for obtaining and characterizing adipose-derived stem cells (ADSCs) from C57BL/6 J mice. The results showed that the yield, viability, and cell morphology obtained differ according to the age of isolated anatomic regions of the adipose tissue from ovarian and epididymis. The results of determination of cyclin D1 showed uniformity in the expression between different populations of ADSCs. A significant increase in the expression of caspase-3 active, was also observed in large cell populations from mice after 120 days. ADSCs were positive for mesenchymal markers CD90 and CD105, Nanog, SSEA-1, CD106, and VEGFR-1, and negative for hematopoietic markers CD34 and CD45. A large number of cells in S + G2/M phases was also observed for both sexes, demonstrating high proliferative capacity of ADSCs. We observed that the adipose tissue of C57BL/6 J mice, isolated from the studied anatomic regions, is a promising source for obtaining pluripotent mesenchymal stem cells with high viability and proliferative response.