Vaginal Histological Changes after Using Intravaginal Sponges for Oestrous Synchronization in Anoestrous Ewes
Vaginal Histological Changes after Using Intravaginal Sponges for Oestrous Synchronization in Anoestrous Ewes
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DOI：
10.1111/rda.12482
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发表时间：
2015-04-01
影响因子：
1.7
通讯作者：
Ungerfeld, R.
中科院分区：
文献类型：
--
作者：
Manes, J.;Campero, C.;Ungerfeld, R.
内容 旨在表征在非配种季节中期使用阴道内海绵（IS）对母羊进行发情同步治疗时所产生的组织学和细胞学阴道变化。将 35 只经产母羊根据取样时间分为三个实验组：（i）用含有 60mg 醋酸甲羟孕酮的 IS 处理 14 天的母羊，在去除 IS 当天取样（ISR 组；n=10），（ii）在发情时或去除海绵后或去除后 72 小时（AR 组；n=14）和（iii）未经海绵处理的母羊，在 IS 去除其他组（CG 组；n=11）当天进行采样。阴道活检和细胞学样本取自阴道前穹窿区域。 CG组的阴道有复层鳞状上皮，固有层有中等程度的淋巴细胞和浆细胞浸润。接受治疗的母羊（ISR 和 AR）出现上皮增生和肥大。 ISR 母羊在 IS 去除时出现出血和血管周围浸润，上皮细胞、中性粒细胞、巨噬细胞和红细胞数量增加。当用于不发情母羊的发情同步时，IS 的使用在阴道壁中产生了组织学和细胞学改变。
Contents To characterize the histological and cytological vaginal changes generated by the use of intravaginal sponge (IS) applied in oestrous synchronization treatments in ewes during mid-non-breeding season. Thirty-five multiparous ewes were allocated to three experimental groups according to the moment in which the samples were taken: (i) ewes treated with IS containing 60mg of medroxyprogesterone acetate for 14days, sampled the day of IS removal (group ISR; n=10), (ii) or after sponge removal at time of oestrus or 72h after removal (group AR; n=14) and (iii) ewes without sponge treatment that were sampled at the day of IS removal of the other groups (group CG; n=11). Vaginal biopsies and cytological samples were taken from the anterior vaginal fornix area. The vagina of the CG group had a stratified squamous epithelium with a moderate degree of cellular infiltration with lymphocytes and plasma cells in the lamina propia. Treated ewes (ISR and AR) had epithelial hyperplasia and hypertrophy. ISR ewes had haemorrhage and perivascular infiltrate and an increased number of epithelial cells, neutrophils, macrophages and erythrocytes at IS removal. The use of IS generated histological and cytological alterations in the vaginal wall when used for oestrous synchronization in anoestrous ewes.