Human mesenchymal stromal cells from adipose tissue of the neck
Human mesenchymal stromal cells from adipose tissue of the neck
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DOI：
10.1007/s00405-012-1943-5
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发表时间：
2012-12-01
影响因子：
2.6
通讯作者：
Kramer, Jan
中科院分区：
文献类型：
--
作者：
Boehrnsen, Florian;Rotter, Nicole;Kramer, Jan
间充质基质细胞（MSC）已被引入组织工程气道移植领域。由于广泛的气管缺损患者通常需要开放式气管切开术，本研究调查了在此过程中是否可以从颈部脂肪组织中获得MSC。通过塑料粘附从8名患者的脂肪组织中分离细胞。分析细胞分离物的（i）增殖，（ii）CD标记物分子的表达和（iii）多谱系分化。分离的梭形细胞表现出高增殖能力，流式细胞仪分析显示符合MSC标准的独特群体。使用经典的MSC培养方案，表征的细胞对于所有分析的细胞分离物显示成脂、成软骨和成骨分化。这项研究能够证明，足够量的干/祖细胞可以很容易地从开放性气管切开术期间获得的颈部脂肪组织中分离出来。这些细胞可能是未来气管替代疗法的来源。
Mesenchymal stromal cells (MSC) have been introduced into the field of tissue-engineered airway transplantation. Since patients with extensive tracheal defects often require an open tracheotomy, this study investigated if MSC could be obtained from the adipose tissue of the neck during this procedure. Cells were isolated by plastic adherence from the adipose tissue of 8 patients. Cell isolates were analyzed for (i) proliferation, (ii) the expression of CD marker molecules and (iii) multilineage differentiation. The isolated spindle-shaped cells showed a high proliferation capacity and the flow cytometric analysis revealed a distinct population meeting the criteria for MSC. Using classical MSC cultivation protocols the characterized cells showed adipogenic, chondrogenic and osteogenic differentiation for all analyzed cell isolates. This study was able to demonstrate that sufficient amounts of stem/progenitor cells can be easily isolated from adipose tissue of the neck obtained during open tracheotomy. These cells may be a source for future tracheal replacement therapies.