Improved 18F labeling of peptides with a fluoride-aluminum-chelate complex.
Improved 18F labeling of peptides with a fluoride-aluminum-chelate complex.
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DOI：
10.1021/bc100137x
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发表时间：
2010-07-21
影响因子：
4.7
通讯作者：
Goldenberg, David M.
中科院分区：
文献类型：
--
作者：
McBride, William J.;D'Souza, Christopher A.;Sharkey, Robert M.;Karacay, Habibe;Rossi, Edmund A.;Chang, Chien-Hsing;Goldenberg, David M.
我们之前报道了使用快速、一锅法用氟 18 (18F) 放射性标记肽的可行性，该方法首先将 18F− 与 Al3+ 混合，然后将 (Al18F)2+ 复合物与肽上的 NOTA 配体结合。在本报告中，我们检查了几种新的 NOTA 配体，并确定了温度、反应时间和试剂浓度如何影响放射性标记产量。在相似的反应条件下，NOTA 配体的四种结构变体的分离放射性标记产率范围为 5.8% 至 87%。所有 Al18F NOTA 复合物在体外人血清中都是稳定的，并且在体内测试的那些复合物也是稳定的。放射性标记反应在 100°C 下进行，肽可在短短五分钟内完成标记。 IMP467 肽的标记浓度高达 115 GBq/μmol (3100 Ci/mmol)，总反应和纯化时间为 30 分钟，无需色谱纯化。
We reported previously the feasibility to radiolabel peptides with fluorine-18 (18F) using a rapid, one-pot, method that first mixes 18F− with Al3+, and then binds the (Al18F)2+ complex to a NOTA ligand on the peptide. In this report, we examined several new NOTA ligands and determined how temperature, reaction time, and reagent concentration affected the radiolabeling yield. Four structural variations of the NOTA ligand had isolated radiolabeling yields ranging from 5.8% to 87% under similar reaction conditions. All of the Al18F NOTA complexes were stable in vitro in human serum and those that were tested in vivo also were stable. The radiolabeling reactions were performed at 100°C and the peptides could be labeled in as little as five minutes. The IMP467 peptide could be labeled up to 115 GBq/μmol (3100 Ci/mmol), with a total reaction and purification time of 30 min without chromatographic purification.
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DOI：
10.1073/pnas.0600982103
发表时间：
2006-05-02
影响因子：
11.1
作者：
Rossi, EA;Goldenberg, DM;Chang, CH
通讯作者：
Chang, CH
影响因子：
4.7
作者：
Chong, Hyun-Soon;Song, Hyun A.;Brechbiel, Martin W.
通讯作者：
Brechbiel, Martin W.
影响因子：
11.5
作者：
Sharkey, RM;Karacay, H;Goldenberg, DM
通讯作者：
Goldenberg, DM
DOI：
10.2967/jnumed.109.066902
发表时间：
2010-03
期刊：
Journal of nuclear medicine : official publication, Society of Nuclear Medicine
影响因子：
--
作者：
Laverman P;McBride WJ;Sharkey RM;Eek A;Joosten L;Oyen WJ;Goldenberg DM;Boerman OC
通讯作者：
Boerman OC
影响因子：
9.3
作者：
McBride, William J.;Sharkey, Robert M.;Goldenberg, David M.
通讯作者：
Goldenberg, David M.