THE POLYPHOSPHOINOSITIDE CYCLE EXISTS IN THE NUCLEI OF SWISS 3T3 CELLS UNDER THE CONTROL OF A RECEPTOR (FOR IGF-I) IN THE PLASMA-MEMBRANE, AND STIMULATION OF THE CYCLE INCREASES NUCLEAR DIACYLGLYCEROL AND APPARENTLY INDUCES TRANSLOCATION OF PROTEIN-KINASE-C TO THE NUCLEUS
THE POLYPHOSPHOINOSITIDE CYCLE EXISTS IN THE NUCLEI OF SWISS 3T3 CELLS UNDER THE CONTROL OF A RECEPTOR (FOR IGF-I) IN THE PLASMA-MEMBRANE, AND STIMULATION OF THE CYCLE INCREASES NUCLEAR DIACYLGLYCEROL AND APPARENTLY INDUCES TRANSLOCATION OF PROTEIN-KINASE-C TO THE NUCLEUS
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DOI：
10.1002/j.1460-2075.1991.tb04883.x
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发表时间：
1991-11-01
期刊：
影响因子：
11.4
通讯作者：
IRVINE, RF
中科院分区：
文献类型：
--
作者：
DIVECHA, N;BANFIC, H;IRVINE, RF
当 Swiss 3T3 细胞用胰岛素样生长因子 I 处理时，细胞核内多磷酸肌醇脂质（磷脂酰肌醇 4-磷酸和磷脂酰肌醇 4,5-二磷酸）的质量迅速减少，同时核二酰甘油增加，蛋白激酶 C 易位至核区域。这与调节肽、铃蟾肽的作用相反，铃蟾肽会在质膜中引起类似的肌醇脂质变化，但对核肌醇水平没有影响，并导致蛋白激酶 C 易位至核后膜。这些结果表明存在一个离散的核多磷酸肌醇信号系统，与众所周知的质膜定位系统完全不同，后者受到细胞表面定位受体的调节控制。
When Swiss 3T3 cells are treated with Insulin-like Growth Factor I, a rapid decrease in the mass of polyphosphoinositol lipids (phosphatidylinositol 4-phosphate and phosphatidylinositol 4,5-bisphosphate) occurs within the nuclei, with a concomitant increase in nuclear diacylglycerol and translocation of protein kinase C to the nuclear region. This is in contrast to the effects of the regulatory peptide, bombesin, which causes similar inositol lipid changes in the plasma membrane, has no effect on nuclear inositide levels and causes a translocation of protein kinase C to post-nuclear membranes. These results suggest the existence of a discrete nuclear polyphosphoinositide signalling system entirely distinct from the well-known plasma membrane-located system, which is under regulatory control by cell surface-located receptors.