NEISSERIA-GONORRHOEAE SURVIVE INTRALEUKOCYTIC OXYGEN-INDEPENDENT ANTIMICROBIAL CAPACITIES OF ANAEROBIC AND AEROBIC GRANULOCYTES IN THE PRESENCE OF PYOCIN LETHAL FOR EXTRACELLULAR GONOCOCCI
NEISSERIA-GONORRHOEAE SURVIVE INTRALEUKOCYTIC OXYGEN-INDEPENDENT ANTIMICROBIAL CAPACITIES OF ANAEROBIC AND AEROBIC GRANULOCYTES IN THE PRESENCE OF PYOCIN LETHAL FOR EXTRACELLULAR GONOCOCCI
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DOI：
10.1128/iai.52.2.384-389.1986
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发表时间：
1986-05-01
影响因子：
3.1
通讯作者：
SPITZNAGEL, JK
中科院分区：
文献类型：
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作者：
CASEY, SG;SHAFER, WM;SPITZNAGEL, JK
研究了淋病奈瑟菌FA 19的一种有纤毛的透明变种对人中性粒细胞（PMN）白细胞内杀伤的抵抗力。在需氧和厌氧PMN单层，约2%的细胞内淋球菌存活长达165分钟。厌氧PMN是有效的需氧PMN在胞内杀死淋球菌。因此，O2-非依赖性的抗菌系统的中性粒细胞在淋球菌的白细胞内杀伤中发挥了重要作用。我们的信念，即幸存的淋球菌是细胞内的支持，通过加入绿脓菌素103消除细胞外细菌，并证实了完整的淋球菌荧光抗体染色后，PMN透性抗体用福尔马林-丙酮处理的PMN单层。我们的数据表明，虽然大多数摄入的淋球菌被O2非依赖性抗菌系统杀死，但即使在小心消除细胞外细菌时，也有少数淋球菌（约2%）存活。
The resistance of a piliated, transparent variant of Neisseria gonorrhoeae FA19 to intraleukocytic killing by human polymorphonuclear neutrophils (PMN) was examined. In both aerobic and anaerobic PMN monolayers, approximately 2% of the intracellular gonococci survived for as long as 165 min. Anaerobic PMN were as effective as aerobic PMN in the intracellular killing of gonococci. Hence, O2-independent antimicrobial systems of PMN performed a significant role in the intraleukocytic killing of gonococci. Our belief that surviving gonococci were intracellular was supported by the elimination of extracellular bacteria by the addition of pyocin 103 and confirmed by the fluorescent antibody staining of intact gonococci after the PMN were permeabilized to antibody with a Formalin-acetone treatment of PMN monolayers. Our data indicate that while the majority of ingested gonococci are killed by O2-independent antimicrobial systems, a small number (about 2%), survive even when care is taken to eliminate extracellular bacteria.