Discovery and Characterization of the Highly Active Fungal Immunomodulatory Protein Fip-vvo82
Discovery and Characterization of the Highly Active Fungal Immunomodulatory Protein Fip-vvo82
复制标题
高活性真菌免疫调节蛋白 Fip-vvo82 的发现和表征
DOI：
10.1021/acs.jcim.6b00087
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发表时间：
2016-10-01
影响因子：
5.6
通讯作者：
Bao，Da-Peng
中科院分区：
文献类型：
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作者：
Wang，Ying;Wang，Ying;Bao，Da-Peng
草菇Volvaria volvacea（Bull. ex Fr.）桑是一种重要的食药用大型真菌，在东亚地区被用于治疗各种疾病已有数百年的历史。鉴定具有独特治疗活性的关键蛋白质。草菇，我们进行了全基因组比较V。通过对volvacea蛋白家族和其他食用菌中缺乏治疗功能的蛋白的研究，鉴定出7种真菌免疫调节蛋白（FIPs）。草菇。基于7个FIPs的生理生化特性预测，推测Fip-vvo 82具有较高的免疫调节活性，并通过分子和免疫学实验证实了这一点，通过三维结构建模和蛋白质-蛋白质对接进一步表征了其特征。这是第一个研究表明，V。volvacea有不止一个FIP。
Volvaria volvacea(Bull. ex Fr.) Sing, an important edible and medicinal macro-fungus, has been used to remedy various diseases for hundreds of years in East Asia. To identify key proteins with the unique therapeutic activity inV. volvacea, we conducted a genomewide comparison ofV. volvaceaprotein families and those of other edible fungi that lack therapeutic functions and identified seven fungal immunomodulatory proteins (FIPs) inV. volvacea. On the basis of the predicted physiological and biochemical characteristics of the seven FIPs, the novel Fip-vvo82 was inferred to have high immunomodulatory activity; this was confirmed by molecular and immunological experiments and further characterized by modeling the three-dimensional structure and protein–protein docking. This is the first study to show thatV. volvaceahas more than one FIP.