MAST-CELL LINES PRODUCE LYMPHOKINES IN RESPONSE TO CROSS-LINKAGE OF FC-EPSILON-RI OR TO CALCIUM IONOPHORES
MAST-CELL LINES PRODUCE LYMPHOKINES IN RESPONSE TO CROSS-LINKAGE OF FC-EPSILON-RI OR TO CALCIUM IONOPHORES
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DOI：
10.1038/339064a0
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发表时间：
1989-05-04
期刊：
影响因子：
64.8
通讯作者：
PAUL, WE
中科院分区：
文献类型：
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作者：
PLAUT, M;PIERCE, JH;PAUL, WE
肥大细胞和嗜碱性粒细胞上的高亲和力Fcε受体(FcεRI)的交联性是诱发变态反应性炎症反应的关键。由于这种交叉连接，肥大细胞分泌各种预先形成的生物活性物质，如组胺和新合成的花生四烯酸代谢物1。在这里，我们展示了FcεRI在一系列未转化的小鼠肥大细胞系上的交叉连接，或用钙离子载体处理这些细胞，刺激信使RNA量的增加和一组典型的由小鼠T细胞系(T2细胞)2产生的淋巴因子的分泌。这些因子包括IL-3(肥大细胞生长因子)S IL-4(一种IgE‘开关因子’)，IL-5(一种嗜酸性细胞分化因子)和IL-6(一种控制免疫球蛋白分泌的因子)。肥大细胞产生的这些多肽因子在诱导过敏和抗寄生虫炎性反应中可能具有重要作用。
THE cross-linkage of high affinity Fcε receptors (FcεRI) on mast cells and basophils is central to the induction of allergic inflammatory responses. As a result of such cross-linkage, mast cells secrete a variety of preformed biologically active substances, such as histamine, and newly synthesized arachidonic acid metabolites1. Here we show that cross-linkage of FcεRI on a series of non-transformed murine mast cell lines, or treatment of these cells with calcium ionophores, stimulates increased messenger RNA levels and secretion of a group of lymphokines classically produced by a subset of murine T cell lines (TH2cells)2. These factors include interleukin-3 (a mast cell growth factor)s interleukin-4 (an IgE 'switch factor'), interleukin-5 (an eosinophil differentiation factor) and interleukin-6 (a factor controlling immunoglobulin secretion). The production of these polypeptide factors by mast cells may have great importance in the induction of allergic and anti-parasite inflammatory responses.