Frequent homozygous deletions in lung cancer cell lines detected by a DNA marker located at 3p21.3-p22.
Frequent homozygous deletions in lung cancer cell lines detected by a DNA marker located at 3p21.3-p22.
复制标题
通过位于 3p21.3-p22 的 DNA 标记检测到肺癌细胞系中频繁的纯合缺失。
作者：
K. Yamakawa;Takashi Takahashi;Y. Horio;Yasushi Murata;Ei;K. Hibi;Shiro Yokoyama;R. Ueda;Y. Nakamura
许多研究已经报道了肺癌中染色体3p的频繁等位基因丢失，我们之前已经证明3p21.3是肺癌和肾细胞癌中常见的缺失区域之一。为了进一步确定含有推定肿瘤抑制基因的区域，我们对26个小细胞肺癌（SCLC）细胞系和10个非小细胞肺癌（NSCLC）细胞系进行了Southern-blot分析，其中40个cosmid标记位于3p21.3-p22。一个标记检测到4个SCLC细胞系和1个NSCLC细胞系的纯合缺失。在这些细胞系中没有其他标记显示纯合缺失或染色体重排。这里描述的纯合子缺失区域估计由小于1兆的DNA组成，并且很可能包含至少一种与肺癌和可能的肾细胞癌的癌变相关的肿瘤抑制基因。
Frequent allelic losses of chromosome 3p in lung cancer have been reported in a number of studies, and we previously demonstrated that 3p21.3 is one of the common regions of deletion in lung cancers and renal cell carcinomas. To further define a region containing the putative tumor suppressor gene, we performed Southern-blot analysis of 26 small cell lung cancer (SCLC) cell lines and ten non-small cell lung cancer (NSCLC) cell lines with 40 cosmid markers located at 3p21.3-p22. One marker detected homozygous deletions of four SCLC cell lines and one NSCLC cell line. None of the other markers revealed homozygous deletions or chromosomal rearrangements in these cell lines. The region of homozygous deletion described here is estimated to consist of less than 1 megabase of DNA, and it is very likely to contain at least one of the tumor suppressor genes associated with carcinogenesis of lung cancer and, possibly, renal cell carcinoma.