NKR-P1+ cells in the rat uterus: granulated metrial gland cells are of the natural killer cell lineage.
NKR-P1+ cells in the rat uterus: granulated metrial gland cells are of the natural killer cell lineage.
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大鼠子宫中的 NKR-P1 细胞：粒状子宫腺细胞属于自然杀伤细胞谱系。
DOI：
10.1095/biolreprod51.3.509
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发表时间：
1994
影响因子：
3.6
通讯作者：
Hiserodt,JC
中科院分区：
文献类型：
--
作者：
Head,JR;Kresge,CK;Young,JD;Hiserodt,JC
母体对妊娠反应的一个有趣的组成部分是大量大颗粒淋巴细胞的分化，称为颗粒子宫腺（GMG）细胞，在蜕膜中，然后在子宫腺中，一个结构在子宫系膜三角独特的啮齿动物妊娠。我们使用了NKR-P1的单克隆抗体3.2.3，NKR-P1是一种参与触发自然杀伤（NK）细胞裂解的表面分子，以确定非妊娠大鼠子宫中NK细胞的数量和分布，以及妊娠期间植入部位的剧烈变化。NKR-P1+细胞在非妊娠子宫中含量丰富，尤其是在子宫内膜中。这些细胞还表达CD 8、CD 2和AsialoGM 1。在上皮下基质中，数量在发情前期和发情期最多;在卵巢切除动物中，它们严重减少，但在补充雌激素后恢复正常。在胚泡附着时（第6天），植入物周围和蜕膜化基质中的NKR-P1+细胞很少。然而，在第8天，大量细胞仅存在于子宫系膜蜕膜中，其中许多细胞表达溶细胞蛋白穿孔素。到第10天，NKR-P1+细胞常见于子宫系膜三角底部的内部肌肉和发育中的子宫腺中，通常含有穿孔素。穿孔素+细胞的数量较多，存在于中央蜕膜朝向外胎盘锥，许多是弱NKR-P1+。在第12天，NKR-P1+细胞几乎完全局限于子宫腺，蜕膜中很少，许多细胞呈弱阳性。观察到更多的含有穿孔素的细胞，表明许多细胞已经失去了可检测的NKR-P1。第6-12天的这种分布模式与GMG细胞的描述相似，表明在大鼠中它们属于NK细胞谱系。这些细胞也是CD 8+和AsialoGM 1+，但CD 2和II类组织相容性抗原阴性，这与它们在形态上相似的白细胞介素-2激活的NK细胞非常不同。参与NK细胞的靶粘附和触发的NKR-P1和CD 2在分化期间的损失与这些细胞报道的不良细胞溶解能力一致。
An intriguing component of the maternal response to pregnancy is the differentiation of large numbers of large granular lymphocytes, termed granulated metrial gland (GMG) cells, in the decidua and then in the metrial gland, a structure in the mesometrial triangle unique to rodent pregnancy. We have used the monoclonal antibody 3.2.3 to NKR-P1, a surface molecule involved in triggering natural killer (NK) cells for lysis, to determine the numbers and distribution of NK cells in the nonpregnant rat uterus and during the dramatic changes at implant sites during pregnancy. NKR-P1+cells were abundant in the nonpregnant uterus, especially in the endometrium. These cells also expressed CD8, CD2, and AsialoGM1. In the subepithelial stroma, the numbers were greatest during proestrus and estrus; in ovariectomized animals, they were severely decreased, but returned to normal with estrogen supplementation. At the time of blastocyst attachment (Day 6), NKR-P1+cells were few around the implant and in the decidualizing stroma. However, on Day 8, substantial numbers were present in the mesometrial decidua only, and many of these cells expressed the cytolytic protein perforin. By Day 10, NKR-P1+cells were common within the inner muscle at the base of the mesometrial triangle and in the developing metrial gland, often containing perforin. Larger numbers of perforin+cells were present in the central decidua towards the ectoplacental cone, and many were weakly NKR-P1+only. On Day 12, NKR-P1+cells were almost completely restricted to the metrial gland, with few in decidua, and many were weakly positive. Substantially more perforin-containing cells were seen, indicating that many had lost detectable NKR-P1. This distribution pattern from Days 6–12 is similar to that described for GMG cells and demonstrates that in the rat they belong to the NK cell lineage. These cells were also CD8+and AsialoGM1+but negative for CD2 and class II histocompatibility antigens, which is very different from interleukin-2-activated NK cells which they resemble morphologically. The loss during differentiation of NKR-P1 and CD2, which are involved in target adhesion and triggering of NK cells, is consistent with the poor cytolytic capacity reported for these cells.