Leukotriene-E4 in human urine: Comparison of on-line purification and liquid chromatography-tandem mass spectrometry to affinity purification followed by enzyme immunoassay
Leukotriene-E4 in human urine: Comparison of on-line purification and liquid chromatography-tandem mass spectrometry to affinity purification followed by enzyme immunoassay
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DOI：
10.1016/j.jchromb.2009.08.011
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发表时间：
2009-10-01
影响因子：
3
通讯作者：
Reisdorph, Nichole
中科院分区：
文献类型：
--
作者：
Armstrong, Michael;Liu, Andrew H.;Reisdorph, Nichole
描述了一种适用于人尿液中 LTE4 高通量测量的新分析方法。该方法利用在线富集和液相色谱/串联质谱 (LC/MS/MS)。这种新型 LC/MS/MS 方法在健康和哮喘受试者的加标尿样中快速且呈线性（5 至 500 pg/ml），并且比酶免疫分析 (EIA) 和之前的 LC/MS/MS 方法更加准确和精密。报告了样本完整性实验的结果以及健康成人和儿童尿液 LTE4 的初步值。 (C) 2009 Elsevier B.V. 保留所有权利。
A new analytical method suitable for high throughput measurements of LTE4 in human urine is described. The methodology utilizes on-line enrichment and liquid chromatography/tandem mass spectrometry (LC/MS/MS). The novel LC/MS/MS method is rapid, linear from 5 to 500 pg/ml in spiked urine samples of both healthy and asthmatic subjects and more accurate and precise than enzyme immunoassay (EIA) and previous LC/MS/MS methods. Results from sample integrity experiments and preliminary values of urinary LTE4 from healthy adults and children are reported. (C) 2009 Elsevier B.V. All rights reserved.