Genetic analysis of forage grasses based on heterologous RFLP markers detected by rice cDNAs
Genetic analysis of forage grasses based on heterologous RFLP markers detected by rice cDNAs
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基于水稻 cDNA 检测的异源 RFLP 标记的饲草遗传分析
DOI：
10.1046/j.1439-0523.2003.00773.x
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发表时间：
2003
期刊：
影响因子：
2
通讯作者：
Tsugutoshi Kishida
中科院分区：
文献类型：
--
作者：
Toshihiko Yamada;Tsugutoshi Kishida
多年生黑麦草(Lolium Perenne L)、高羊茅(Festuca pratensis Huds.)和高羊茅(Festuca arundinacea Schreb.)利用197个水稻基因克隆与牧草基因组进行杂交。许多水稻克隆没有可见的条带，或者只有一条涂片，没有离散的条带。23个克隆与牧草基因组DNA进行了高效杂交。利用12个多态的水稻cDNARFLP探针，对5个牧草品种和1个群体的遗传变异进行了分析。根据Rogers的遗传距离衡量，草地早熟禾品种‘Tomosakae’的品种内遗传变异性显著低于多年生羊茅和高羊茅品种。为了确定不同物种之间的遗传多样性，利用12个RFLP探针的数据进行了聚类分析。多年生黑麦草的两个变种比高羊茅的三个变种聚在一起更紧密。高羊茅的两个日本变种聚在一起。多年生草本植物、草地早熟禾和高羊茅的6个种质间存在品种特异性的RFLP标记。这种品种特异的RFLP标记将为黑麦属和高羊茅属的属间杂交等育种计划提供非常有用的工具。
Genetic polymorphism within and between three species of forage grasses, perennial ryegrass (Lolium perenne L), meadow fescue (Festuca pratensis Huds.) and tall fescue (Festuca arundinacea Schreb.), was analyzed using restriction fragment length polymorphism (RFLP) markers detected by rice cDNA probes developed at the Rice Genome Research Programme of Japan (RGP). One hundred and ninety-seven rice cDNA clones were used for hybridization to genomic DNA of forage grasses. Many of the rice cDNA clones produced no visible band or only a smear with no discrete bands. Twenty-three clones showed high efficiency cross-hybridization to the genomic DNA of forage grasses. Genetic variation was evaluated for five varieties and one population of forage grasses using 12 polymorphic rice cDNA RFLP probes. Genetic variability within varieties as measured by Rogers' genetic distance was considerably lower for the F. pratensis variety 'Tomosakae' than for the L. perenne and F. arundinacea varieties. To determine the genetic diversity between varieties of different species, cluster analysis was performed using data from the 12 RFLP probes. The two accessions of Lolium perenne were clustered more closely together than the three varieties of F. arundinacea. Two Japanese varieties of F. arundinacea were grouped in the same cluster. The variety-specific RFLP markers were seen among six accessions of L. perenne, F. pratensis and F. arundinacea. Such variety-specific RFLP markers would provide very useful tools for breeding programmes such as the intergeneric hybridization of Lolium and Festuca genera.