An MYB transcription factor from Malus xiaojinensis has a potential role in iron nutrition.
An MYB transcription factor from Malus xiaojinensis has a potential role in iron nutrition.
复制标题
DOI：
10.1111/j.1744-7909.2008.00761.x
复制
发表时间：
2008-10
影响因子：
11.4
通讯作者：
Jie Shen;Xuefeng Xu;Tianzhong Li;Dongmei Cao;Zhenhai Han
中科院分区：
文献类型：
--
作者：
Jie Shen;Xuefeng Xu;Tianzhong Li;Dongmei Cao;Zhenhai Han
铁的吸收和利用的调控对植物的生存和生长至关重要。我们从小金海棠中分离到一个MYB基因，命名为MxMYB1，该基因是在缺铁条件下诱导的。缺铁条件下，MxMYB1基因在根中表达上调，但在叶中表达不明显，提示MxMYB1可能参与了根中的铁营养。在铁正常(40微米)和缺铁(缺铁300微米)条件下，转MxMYB1基因拟南芥植株的铁含量均低于野生型植株。但转基因植株中铜、锌、锰的含量并未发生变化。基因芯片和实时定量聚合酶链式反应分析表明，两个铁相关基因的表达可能受到MxMYB1的负调控，因为在铁正常和缺铁条件下，MxMYB1转基因拟南芥中编码铁转运蛋白AtIRT1和铁储存蛋白AtFER1的基因表达水平低于野生型植株。这些结果表明，MxMYB1可能是植物铁吸收和储存的负调控因子。
Regulation of iron uptake and use is critical for plant survival and growth. We isolated an MYB gene from Malus xiaojinensis named MxMYB1, which is induced under Fe-deficient conditions. Expression of MxMYB1 was upregulated by Fe starvation in the roots but not in leaves, suggesting that MxMYB1 might play a role in iron nutrition in roots. Transgenic Arabidopsis plants expressing MxMYB1 exhibited lower iron content as compared with wild type plants under both Fe-normal (40 microM) and Fe-deficient conditions (Fe omitted and Ferrozine 300 microM). However, the contents of Cu, Zn and Mn were not changed in these transgenic plants. Gene chip and real-time polymerase chain reaction analyses indicated that the expression of two Fe-related genes encoding an iron transporter AtIRT1 and an iron storage protein ferritin AtFER1 might be negatively regulated by MxMYB1 as the expression levels of these genes were lower in MxMYB1 expressing transgenic Arabidopsis plants as compared with wild type plants under both Fe-normal and Fe-deficient conditions. These results suggest that MxMYB1 may function as a negative regulator of iron uptake and storage in plants.