Rpp2, an essential protein subunit of nuclear RNase P, is required for processing of precursor tRNAs and 35S precursor rRNA in Saccharomyces cerevisiae.
Rpp2, an essential protein subunit of nuclear RNase P, is required for processing of precursor tRNAs and 35S precursor rRNA in Saccharomyces cerevisiae.
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DOI：
10.1073/pnas.95.12.6716
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发表时间：
1998-06
影响因子：
11.1
通讯作者：
V. Štolc;A. Katz;S. Altman
中科院分区：
文献类型：
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作者：
V. Štolc;A. Katz;S. Altman
RPP2是在酿酒酵母基因组中发现的一个必需基因，编码核核糖核酸酶P的15.8 kDa蛋白亚基。通过与人蛋白Rpp20的序列相似性检测到RPP2，Rpp20与人RNaseP共同提纯。在细胞粗提物的免疫沉淀物中，可以发现表位标记的RPP2与RNaseP和RNase线粒体RNA的加工有关。RPP2蛋白在体内的耗尽会导致含有未加工内含子以及5‘和3’末端的前体tRNAs的积累，并导致35S前体rRNA的加工缺陷。RPP2缺失的细胞在处理5.8S rRNA的过程中存在缺陷。RPP2免疫沉淀物准确地切割酵母前体tRNAs和前体rRNAs，并包含人类硬皮病自身免疫抗原Rpp30的Rpp1蛋白同源物。这些结果表明，RPP2是核核糖核酸酶P的一个蛋白亚基，在从酵母到人类的真核生物中都是功能保守的。
RPP2, an essential gene that encodes a 15.8-kDa protein subunit of nuclear RNase P, has been identified in the genome of Saccharomyces cerevisiae. Rpp2 was detected by sequence similarity with a human protein, Rpp20, which copurifies with human RNase P. Epitope-tagged Rpp2 can be found in association with both RNase P and RNase mitochondrial RNA processing in immunoprecipitates from crude extracts of cells. Depletion of Rpp2 protein in vivo causes accumulation of precursor tRNAs with unprocessed introns and 5' and 3' termini, and leads to defects in the processing of the 35S precursor rRNA. Rpp2-depleted cells are defective in processing of the 5.8S rRNA. Rpp2 immunoprecipitates cleave both yeast precursor tRNAs and precursor rRNAs accurately at the expected sites and contain the Rpp1 protein orthologue of the human scleroderma autoimmune antigen, Rpp30. These results demonstrate that Rpp2 is a protein subunit of nuclear RNase P that is functionally conserved in eukaryotes from yeast to humans.