BINDING OF ALPHA-FACTOR PHEROMONE TO YEAST A-CELLS - CHEMICAL AND GENETIC-EVIDENCE FOR AN ALPHA-FACTOR RECEPTOR
BINDING OF ALPHA-FACTOR PHEROMONE TO YEAST A-CELLS - CHEMICAL AND GENETIC-EVIDENCE FOR AN ALPHA-FACTOR RECEPTOR
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DOI：
10.1016/0092-8674(83)90186-1
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发表时间：
1983-01-01
期刊：
影响因子：
64.5
通讯作者：
HARTWELL, LH
中科院分区：
文献类型：
--
作者：
JENNESS, DD;BURKHOLDER, AC;HARTWELL, LH
酵母菌a细胞的分裂周期被α因子抑制。单倍体A细胞结合35S标记的α因子，而单倍体α结合。细胞和二倍体α/α。细胞几乎没有结合。α-因子与细胞的关联在稀释后是可逆的。未标记的α-因子竞争结合35S-α-因子；竞争的浓度依赖关系显示9倍。每个细胞105个结合位点，Kd值为3倍。10~(-7)M。缔合率(K_n=3。103M~(-1)·S~(-1))和解离(Koff=9次)。10~(-4)S~(-1))，符合平衡常数。与5个温度敏感的ste2突变体相关的α因子结合活性是不耐热的，这表明STE2基因编码α因子的受体。相反，其他温度敏感突变体(ste4、ste5、ste7、ste11和stE12)的结合活性不表现出耐热性。
The division cycle of yeast a cells is inhibited by .alpha.-factor. Haploid a cells bound 35S-labeled .alpha.-factor, whereas haploid .alpha. cells and diploid a/.alpha. cells showed little binding. The association of .alpha.-factor with a cells was reversible upon dilution. Unlabeled .alpha.-factor competed for binding of 35S-.alpha.-factor; the concentration dependence for competition indicated 9 .times. 105 binding sites per cell with a Kd of 3 .times. 10-7 M. The rates of association (kon = 3 .times. 103 M-1 s-1) and dissociation (koff = 9 .times. 10-4 s-1) were consistent with the equilibrium constant. The .alpha.-factor binding activity associated with 5 temperature-sensitive ste2 mutants was thermolabile, suggesting that the STE2 gene encodes the receptor for .alpha.-factor. In contrast, the binding activity of other temperature-sensitive mutants (ste4, ste5, ste7, ste11 and ste12) showed no thermolability.