Efficient Shoot Regeneration System of Pear Rootstock OHF 333 (Pyrus communis L.) Leaves
Efficient Shoot Regeneration System of Pear Rootstock OHF 333 (Pyrus communis L.) Leaves
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DOI：
10.17660/actahortic.2009.839.23
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发表时间：
2009-07
期刊：
影响因子：
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通讯作者：
L. Nacheva;P. Gercheva;V. Dzhuvinov
中科院分区：
文献类型：
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作者：
L. Nacheva;P. Gercheva;V. Dzhuvinov
梨砧木OH × F（P. communis L.）在美国培育，比BA 29生长旺盛10%，但比“威廉姆斯”幼苗生长缓慢。该砧木的特点是高产效率和中等抗火疫病（欧文氏菌）。OH × F的一些无性系很难繁殖，这就是为什么它们在体外繁殖的原因。以梨砧木OHF 333叶片为外植体，建立了高效的不定芽再生体系，并研究了植物生长调节剂对不定芽再生能力的影响。研究了OHF 333（P. communis L.）品种将来源植株的叶片切段培养在基于Murashige和Skoog（MS）的营养培养基上进行再生，所述营养培养基添加TDZ（噻苯隆，7.5和9μM）、2.46 μM吲哚-3-丁酸（IBA）、10 g/L蔗糖、30 g/L山梨醇和不同含量的吲哚乙酸（IAA）（5、10、15和20 μM）。将外植体在黑暗中培养15天，然后在22±2°C的温度下在16/8小时的光周期（40 μmol m-2 s-1 PPFD）下培养20天。以TDZ + IBA + IAA（7.5 μM +2.46 μM +20 μM）和TDZ + IBA + IAA（9 μM +2.46 μM +10 μM）两种培养基的体细胞器官发生率最高，达80%以上，每个外植体再生5个以上。将获得的所有再生体进行克隆、繁殖，并在驯化后进行对火疫病（梨火疫病菌）的抗性测试。
The pear rootstock OH × F (P. communis L.), bred in the USA, shows 10% more vigorous growth compared to BA29, but it has a slower growth than the seedlings of ‘Williams’. The rootstock is characterized by high yield efficiency and moderate resistance to fire blight (Erwinia amylovora). Some clones of OH × F are difficult to propagate, that is why they are propagated in vitro. The aim of the present research was to develop an efficient system for shoot regeneration from pear rootstock OHF 333 leaf explants and to investigate the effect of plant growth regulators on the regeneration capacity. Studies were carried out with leaf segments of in vitro propagated plants of OHF 333 (P. communis L.) cultivar. Leaf segments of the source plants were cultivated on nutrient media for regeneration based on Murashige and Skoog (MS) with added TDZ (thidiazuron, 7.5 and 9μM), 2.46 μM indole-3-butyric acid (IBA), 10 g/L sucrose, 30 g/L sorbitol and different indole acetic acid (IAA) content - 5, 10, 15 and 20 μM. The explants were cultivated in darkness for 15 days, after which at a photoperiod of 16/8 hours (40 μmol m -2 s -1 PPFD) at a temperature of 22±2°C for 20 days. The best efficiency of somatic organogenesis (over 80% regeneration and more than 5 regenerants per explant) was achieved on two nutrient media - with 7.5 μM TDZ, 2.46 μM IBA and 20 μM IAA or with 9 μM TDZ, 2.46 μM IBA and 10 μM IAA. All the regenerants obtained were cloned, propagated and would be tested for resistance to fire blight (Erwinia amylovora) after acclimatization.