Protein Compositions Changes of Circulating Microparticles in Patients With Valvular Heart Disease Subjected to Cardiac Surgery Contribute to Systemic Inflammatory Response and Disorder of Coagulation
Protein Compositions Changes of Circulating Microparticles in Patients With Valvular Heart Disease Subjected to Cardiac Surgery Contribute to Systemic Inflammatory Response and Disorder of Coagulation
复制标题
接受心脏手术的瓣膜性心脏病患者循环微粒的蛋白质组成变化导致全身炎症反应和凝血障碍
DOI：
10.1097/shk.0000000000001309
复制
发表时间：
2019-11-01
期刊：
影响因子：
3.1
通讯作者：
Ou, Jing-Song
中科院分区：
文献类型：
--
作者：
Jian, Yu-Peng;Yuan, Hao-Xiang;Ou, Jing-Song
我们最近证明，接受心脏手术的瓣膜性心脏病（VHD）患者的循环微粒（MP）会损害内皮功能和血管舒张。然而，尚不清楚这些循环 MP 的蛋白质组成是否实际上因疾病和手术而发生变化。在心脏手术前和术后 72 小时，从年龄匹配的对照受试者 (n = 50) 和 VHD 患者 (n = 50) 中分离出循环 MP。通过液相色谱和质谱结合同量异位标签的相对和绝对定量技术进行蛋白质组学研究。通过 ProteinPilot 鉴定差异蛋白，其中一些蛋白通过蛋白质印迹法验证。对差异蛋白进行生物信息分析。总共鉴定了 849 个蛋白质，并且在所有三组中都发现了 453 个蛋白质。同时，对照组、术前组和术后组分别有 165、39 和 80 个蛋白质是独特的。这些独特的蛋白质在定位、分子功能和生物过程方面都不同。 VHD 患者的促炎蛋白增加，术后更是如此。手术前后与凝血相关的蛋白质发生了巨大变化。接受心脏手术的 VHD 患者循环 MP 的蛋白质组成发生改变，这可能导致全身炎症反应激活和凝血障碍。
We recently demonstrated that circulating microparticles (MPs) from patients with valvular heart diseases (VHD) subjected to cardiac surgery impaired endothelial function and vasodilation. However, it is unknown whether or not the protein composition of these circulating MPs actually changes in response to the disease and the surgery. Circulating MPs were isolated from age-matched control subjects (n = 50) and patients (n = 50) with VHD before and 72 h after cardiac surgery. Proteomics study was performed by liquid chromatography and mass spectrometry combined with isobaric tags for relative and absolute quantification technique. The differential proteins were identified by ProteinPilot, some of which were validated by Western blotting. Bio-informatic analysis of differential proteins was carried out. A total of 849 proteins were identified and 453 proteins were found in all three groups. Meanwhile, 165, 39, and 80 proteins were unique in the control, pre-operation, and postoperation groups respectively. The unique proteins were different in localization, molecular function, and biological process. The pro-inflammatory proteins were increased in VHD patients and more so postoperatively. Proteins related to coagulation were dramatically changed before and after surgery. The protein composition of circulating MPs was changed in patients with VHD undergoing cardiac surgery, which may lead to activation of the systemic inflammatory response and disorders of coagulation.