In vivo label-free three-dimensional imaging of zebrafish vasculature with optical projection tomography
In vivo label-free three-dimensional imaging of zebrafish vasculature with optical projection tomography
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DOI：
10.1117/1.3640808
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发表时间：
2011-10-01
影响因子：
3.5
通讯作者：
Valentini, Gianluca
中科院分区：
文献类型：
--
作者：
Bassi, Andrea;Fieramonti, Luca;Valentini, Gianluca
我们介绍了流动光学投影层析成像技术，这是一种能够在3-D中显示活体标本血管系统的成像技术。该方法检测血流中细胞的运动，并使用运动分析程序创建流程图。然后，通过从不同角度拍摄的投影获得的流图被用于重建标本的循环系统的部分。因此，我们展示了一种活体三维光学成像技术，该技术无需任何标记，即可重建和可视化透明和弱散射的活体标本的血管网络。(C)2011年，光学仪器工程师学会。[DOI：10.1117/1.3640808]
We introduce flow optical projection tomography, an imaging technique capable of visualizing the vasculature of living specimens in 3-D. The method detects the movement of cells in the bloodstream and creates flow maps using a motion-analysis procedure. Then, flow maps obtained from projection taken at several angles are used to reconstruct sections of the circulatory system of the specimen. We therefore demonstrate an in vivo, 3-D optical imaging technique that, without the use of any labeling, is able to reconstruct and visualize the vascular network of transparent and weakly scattering living specimens. (C) 2011 Society of Photo-Optical Instrumentation Engineers (SPIE). [DOI: 10.1117/1.3640808]