Effects of milk product intake on thigh muscle strength and NFKB gene methylation during home-based interval walking training in older women: A randomized, controlled pilot study.
Effects of milk product intake on thigh muscle strength and NFKB gene methylation during home-based interval walking training in older women: A randomized, controlled pilot study.
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DOI：
10.1371/journal.pone.0176757
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Nose H
中科院分区：
文献类型：
--
作者：
Masuki S;Nishida K;Hashimoto S;Morikawa M;Takasugi S;Nagata M;Taniguchi S;Rokutan K;Nose H
随着年龄的增长，肌肉萎缩与慢性全身性炎症和生活方式相关疾病密切相关。在本研究中，我们评估了在5个月间歇步行训练（IWT）期间运动后奶制品摄入是否能增强老年妇女大腿肌肉力量并改善对炎症的易感性。参加本研究的受试者[n = 37, 66±5（标准差）年]均为实施内力治疗60个月。他们被随机分为以下3组：单独IWT组（CNT, n = 12）， IWT +运动后低剂量乳制品摄入组（LD, n = 12； 4 g蛋白质和3 g碳水化合物）或IWT +比LD组高3倍剂量的乳制品摄入组（HD, n = 13）。他们被要求在步行峰值有氧能力≥70%和40%时，每天重复≥5组快走和慢走，每组3分钟，持续≥4天/周。IWT后，HD组大腿肌肉力量的增加（8±2%）高于CNT组（-2±3%,P = 0.022），尽管两组之间的IWT效果相似（P = 0.15）。全血焦磷酸测序分析显示，HD组炎症主控基因NFKB1和NFKB2的甲基化水平（分别为29±7%和44±11%）高于CNT组（分别为-20±6%和-10±6%，P<0.001）。此外，全基因组DNA甲基化分析显示，与CNT组相比，HD组中几个炎症相关基因被超甲基化，这表明HD组中促炎细胞因子基因受到更大的抑制。在体力活动的老年妇女中，运动后摄入HD奶制品使大腿肌肉力量和NFKB1和NFKB2基因甲基化增加了更大的百分比。UMIN-CTR没有。UMIN000024544和编号UMIN000024912
Muscle atrophy with aging is closely associated with chronic systemic inflammation and lifestyle-related diseases. In the present study, we assessed whether post-exercise milk product intake during 5-month interval walking training (IWT) enhanced the increase in thigh muscle strength and ameliorated susceptibility to inflammation in older women. Subjects [n = 37, 66±5 (standard deviation) yrs] who had been performing IWT for >6 months participated in this study. They were randomly divided into the following 3 groups: IWT alone (CNT, n = 12), IWT + low-dose post-exercise milk product intake (LD, n = 12; 4 g protein and 3 g carbohydrate) or IWT + a 3-times higher dose of milk product intake than the LD group (HD, n = 13). They were instructed to repeat ≥5 sets of fast and slow walking for 3 min each at ≥70% and 40% peak aerobic capacity for walking, respectively, per day for ≥4 days/week. After IWT, thigh muscle strength increased in the HD group (8±2%) more than in the CNT group (-2±3%, P = 0.022), despite similar IWT achievements between the groups (P>0.15). Pyrosequencing analysis using whole blood showed that methylation of NFKB1 and NFKB2, master genes of inflammation, was enhanced in the HD group (29±7% and 44±11%, respectively) more than in the CNT group (-20±6% and -10±6%, respectively; P<0.001). Moreover, the genome-wide DNA methylation analysis showed that several inflammation-related genes were hyper-methylated in the HD group compared with that in the CNT group, suggesting greater pro-inflammatory cytokine gene suppression in the HD group. HD milk product intake after exercise produced a greater percent increase in thigh muscle strength and NFKB1 and NFKB2 gene methylation during IWT in physically active older women. UMIN-CTR No. UMIN000024544 and No. UMIN000024912