Heterogeneity of estrogen binding sites in mouse mammary cancer.
Heterogeneity of estrogen binding sites in mouse mammary cancer.
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小鼠乳腺癌中雌激素结合位点的异质性。
DOI：
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发表时间：
1980
期刊：
影响因子：
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通讯作者：
J. H. Clark
中科院分区：
文献类型：
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作者：
C. Watson;J. H. Clark
最近在我们的实验室中证实，大鼠子宫中至少有两个特异性雌激素结合位点，这促使我们研究可移植卵巢依赖性小鼠乳腺肿瘤（MXT-3590）中结合位点的相似异质性。细胞质（硫酸鱼精蛋白或羟基磷灰石交换试验）或粗核部分（硫酸鱼精蛋白沉淀核交换试验）的饱和度分析显示两个结合组分：I型，符合经典描述的雌激素受体和II型，具有较低的亲和力雌二醇，但比I型网站更大的能力。暴露于活性炭的胞质溶胶从II型位点部分去除结合的3 H-雌二醇，但不从I型位点去除结合的3 H-雌二醇。II型位点对雌激素是特异性的，并且不从细胞质易位到核隔室。虽然II型网站进行解离预标记蔗糖密度梯度，他们很容易证明后标记蔗糖密度梯度馏分和…
AbstractThe recent demonstration in our laboratory of at least two specific estrogen binding sites in the rat uterus prompted us to investigate similar heterogeneity of binding sites in a trans-plantable ovarian dependent mouse mammary tumor (MXT-3590). Saturation analysis of cytoplasmic (protamine sulfate or hydroxylapatite exchange assay) or crude nuclear fractions (protamine sulfate precipitated nuclear exchange assay) revealed two binding components: type I which conforms to the classically described estrogen receptor and type II which has a lower affinity for estradiol but a greater capacity than type I sites. Exposure of cytosol to charcoal partially removes bound 3H-estradiol from type II sites but not from type I sites. Type II sites are specific for estrogens and do not translocate from the cytoplasmic to the nuclear compartment. Although Type II sites undergo dissociation on prelabeled sucrose density gradients, they are readily demonstrable by postlabeling sucrose density gradient fractions and...