Effect of amplitude and frequency of cyclic tensile strain on the inhibition of MMP-1 mRNA expression in tendon cells: An in vitro study
Effect of amplitude and frequency of cyclic tensile strain on the inhibition of MMP-1 mRNA expression in tendon cells: An in vitro study
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DOI：
10.1080/03008200390215881
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发表时间：
2003-05-01
影响因子：
2.9
通讯作者：
Vaupel, Z
中科院分区：
文献类型：
--
作者：
Lavagnino, M;Arnoczky, SP;Vaupel, Z
确定循环应变振幅和频率对MMP-1的影响（间质胶原酶）的表达，大鼠尾腱（RTT）被固定或循环移位到不同的幅度（0.017 Hz时的1、3或6%应变）或频率（1%应变，0.017，0.17，或1.0 Hz）24小时。应激剥夺24小时导致MMP-1表达显著上调。发现0.017 Hz的循环拉伸载荷显著抑制，但不能完全消除，MMP-1在1%应变下的表达。在3%和6%应变下，MMP-1表达完全消除。将施加1%应变的频率增加到0.17或1.0 Hz完全消除MMP-1表达。用细胞松弛素D破坏肌动蛋白细胞骨架，取消了周期性应变对MMP-1表达的所有抑制作用。我们的研究结果表明，MMP-1在肌腱细胞中的表达可以调制不同的振幅和频率的周期性拉伸应变，大概是通过一个基于细胞的mechanotransduction途径。
To determine the effect of cyclic strain amplitude and frequency on MMP-1 (interstitial collagenase) expression in tendon cells, rat tail tendons (RTT) were immobilized or cyclically displaced to various amplitudes (1, 3, or 6% strain at 0.017 Hz) or frequencies (1% strain at 0.017, 0.17, or 1.0 Hz) for 24 hr. Stress-deprivation for 24 hr resulted in a marked upregulation in MMP-1 expression. Cyclic tensile loading at 0.017 Hz was found to significantly inhibit, but not completely eliminate, MMP-1 expression at 1% strain. MMP-1 expression was completely eliminated at 3 and 6% strain. Increasing the frequency of application of the 1% strain to 0.17 or 1.0 Hz completely eliminated MMP-1 expression. Disruption of the actin cytoskeleton with cytochalasin D abolished all inhibitory effects of cyclic strain on MMP-1 expression. The results of our study demonstrate that MMP-1 expression in tendon cells can be modulated by varying amplitudes and frequencies of cyclic tensile strain, presumably through a cytoskeletally based mechanotransduction pathway.